CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021079
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bifunctional protein NCOAT 
Protein Synonyms/Alias
 Meningioma-expressed antigen 5; Nuclear cytoplasmic O-GlcNAcase and acetyltransferase; Protein O-GlcNAcase; Glycoside hydrolase O-GlcNAcase; Hexosaminidase C; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; O-GlcNAcase; OGA; Histone acetyltransferase; HAT 
Gene Name
 Mgea5 
Gene Synonyms/Alias
 Hexc; Kiaa0679 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
240YLRTVGEKLLPGIEVubiquitination[1]
297RLFLGPYKGRSTELIubiquitination[1]
723TIRPYFPKDEASVYKubiquitination[1]
730KDEASVYKICREMYDubiquitination[1]
869ACLLSSLKANGSRGAubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Cleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl- beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Possesses hyaluronidase activity (By similarity). Acetylates 'Lys-8' of histone H4. 
Sequence Annotation
 REGION 583 916 Histone acetyltransferase activity (By
 REGION 695 814 Required for histone H4 binding.
 ACT_SITE 175 175 Nucleophile; for O-GlcNAcase activity.
 ACT_SITE 177 177 Proton donor; for O-GlcNAcase activity.
 MOD_RES 364 364 Phosphoserine.
 DISULFID 777 793  
Keyword
 Acyltransferase; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Disulfide bond; Glycosidase; Hydrolase; Multifunctional enzyme; Nucleus; Phosphoprotein; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 916 AA 
Protein Sequence
MVQKESQAAL EERESERNAN PAAASGASLE QSVAPAPGED NPSGAGAAAV VGAAGGARRF 60
LCGVVEGFYG RPWVMEQRKE LFRRLQKWEL NTYLYAPKDD YKHRMFWREM YSVEEAEQLM 120
TLISAAREYE IEFIYAISPG LDITFSNPKE VSTLKRKLDQ VSQFGCRSFA LLFDDIDHNM 180
CAADKEVFSS FAHAQVSITN EIYQYLGEPE TFLFCPTEYC GTFCYPNVSQ SPYLRTVGEK 240
LLPGIEVLWT GPKVVSKEIP VESIEEVSKI IKRAPVIWDN IHANDYDQKR LFLGPYKGRS 300
TELIPRLKGV LTNPNCEFEA NYVAIHTLAT WYKSNMNGVR KDVVMTDSED STVSIQIKLE 360
NEGSDEDIET DVLYSPQMAL KLALTEWLQE FGVPHQYSSR QVAHSGAKTS VVDGTPLVAA 420
PSLNATTVVT TVYQEPIMSQ GAALSGEPSV LTKEEEKKQP DEEPMDMVVE KQEEAEHKND 480
NQILTEIVEA KMAEELRPMD TDKESMAESK SPEMSMQEDC IPDVAPMQTD EQTQKEQFVP 540
GPNEKPLYTA EPVTLEDLQL LADLFYLPYE HGPKGAQMLR EFQWLRANSS VVSVNCKGKD 600
SEKIEEWRSR AAKFEEMCAL VMGMFTRLSN CANRTILYDM YSYVWDIKSI MSMVKSFVQW 660
LGCRSHSSAQ FLIGDQEPWA FRGGLAGEFQ RLLPIDGAND LFFQPPPLTP TSKVYTIRPY 720
FPKDEASVYK ICREMYDDGV GFPFQSQPDL IGDKLVGGLL SLSLDYCFVL EDEDGICGYA 780
LGTVDVTPFI KKCKISWIPF MQEKYTKPNG DKELSEAEKI MLSFHEEQEV LPETFLANFP 840
SLIKMDIHKK VTDPSVAKSM MACLLSSLKA NGSRGAFCEV RPDDKRILEF YSKLGCFEIA 900
KMEGFPKDVV ILGRSL 916 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004402; F:histone acetyltransferase activity; IEA:EC.
 GO:0016798; F:hydrolase activity, acting on glycosyl bonds; IEA:UniProtKB-KW.
 GO:0007568; P:aging; IEA:Compara.
 GO:0046060; P:dATP metabolic process; IEA:Compara.
 GO:0006044; P:N-acetylglucosamine metabolic process; IEA:Compara.
 GO:0070265; P:necrotic cell death; IEA:Compara.
 GO:0010616; P:negative regulation of cardiac muscle adaptation; IEA:Compara.
 GO:0060051; P:negative regulation of protein glycosylation; IEA:Compara.
 GO:0010524; P:positive regulation of calcium ion transport into cytosol; IEA:Compara.
 GO:0031343; P:positive regulation of cell killing; IEA:Compara.
 GO:0051054; P:positive regulation of DNA metabolic process; IEA:Compara.
 GO:0046326; P:positive regulation of glucose import; IEA:Compara.
 GO:0060124; P:positive regulation of growth hormone secretion; IEA:Compara.
 GO:0032024; P:positive regulation of insulin secretion; IEA:Compara.
 GO:0051901; P:positive regulation of mitochondrial depolarization; IEA:Compara.
 GO:0043243; P:positive regulation of protein complex disassembly; IEA:Compara.
 GO:0045862; P:positive regulation of proteolysis; IEA:Compara.
 GO:0006612; P:protein targeting to membrane; IEA:Compara.
 GO:0048545; P:response to steroid hormone stimulus; IEA:Compara. 
Interpro
 IPR016181; Acyl_CoA_acyltransferase.
 IPR011496; Beta-N-acetylglucosaminidase.
 IPR017853; Glycoside_hydrolase_SF. 
Pfam
 PF07555; NAGidase 
SMART
  
PROSITE
  
PRINTS