UniProt Accession

 SEC14_YEAST; P24280; D6VZQ3 

Genbank Protein ID

 X15483; Z49259; BK006946 

Genbank Nucleotide ID

 CAA33511.1; CAA89225.1; DAA09977.1 

Protein Name

 SEC14 cytosolic factor 

Protein Synonyms/Alias

 Phosphatidylinositol/phosphatidylcholine transfer protein; PI/PC TP 

Gene Name

 SEC14 

Gene Synonyms/Alias

 PIT1; YMR079W; YM9582.04 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
7	*MVTQQEKEFLESYP	ubiquitination	[1]
42	KALAELRKLLEDAGF	ubiquitination	[1, 2, 3]
66	LRFLRARKFDVQLAK	acetylation	[4]
84	ENCEKWRKDYGTDTI	ubiquitination	[1, 3]
145	TSEERMLKNLVWEYE	ubiquitination	[1]
229	STAFRLFKPFLDPVT	acetylation	[4]
249	ILGSSYQKELLKQIP	acetylation	[4]
253	SYQKELLKQIPAENL	acetylation	[4]
267	LPVKFGGKSEVDESK	acetylation	[4]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis.
 Ziv I, Matiuhin Y, Kirkpatrick DS, Erpapazoglou Z, Leon S, Pantazopoulou M, Kim W, Gygi SP, Haguenauer-Tsapis R, Reis N, Glickman MH, Kleifeld O.
 Mol Cell Proteomics. 2011 May;10(5):M111.009753. [PMID: 21427232]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [4] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Required for transport of secretory proteins from the Golgi complex. Catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes in vitro. Essential for viability and secretion. 

Sequence Annotation

 DOMAIN 99 272 CRAL-TRIO.
 MOD_RES 302 302 Phosphoserine.  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; Golgi apparatus; Membrane; Phosphoprotein; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 304 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:SGD.
 GO:0000139; C:Golgi membrane; IDA:SGD.
 GO:0008525; F:phosphatidylcholine transporter activity; IDA:SGD.
 GO:0008526; F:phosphatidylinositol transporter activity; IDA:SGD.
 GO:0030437; P:ascospore formation; IMP:SGD.
 GO:0043001; P:Golgi to plasma membrane protein transport; IMP:SGD.
 GO:0006896; P:Golgi to vacuole transport; IMP:SGD.
 GO:0048194; P:Golgi vesicle budding; IDA:SGD.
 GO:2001246; P:negative regulation of phosphatidylcholine biosynthetic process; IMP:SGD.
 GO:1901352; P:negative regulation of phosphatidylglycerol biosynthetic process; IMP:SGD.
 GO:0046488; P:phosphatidylinositol metabolic process; IMP:SGD. 

Interpro

 IPR001071; CRAL-bd_toc_tran.
 IPR001251; CRAL-TRIO_dom.
 IPR011074; CRAL/TRIO_N_dom. 

Pfam

 PF00650; CRAL_TRIO
 PF03765; CRAL_TRIO_N 

SMART

 SM01100; CRAL_TRIO_N
 SM00516; SEC14 

PROSITE

 PS50191; CRAL_TRIO 

PRINTS

 PR00180; CRETINALDHBP.