UniProt Accession

 PDIA6_HUMAN; Q15084; Q53RC7; Q6ZSH5; Q99778 

Genbank Protein ID

 D49489; AK127433; AK289428; AC092687; CH471053; BC001312; U79278 

Genbank Nucleotide ID

 BAA08450.1; BAC86977.1; BAF82117.1; AAY24070.1; EAX00950.1; AAH01312.1; AAB50217.1 

Protein Name

 Protein disulfide-isomerase A6 

Protein Synonyms/Alias

 Endoplasmic reticulum protein 5; ER protein 5; ERp5; Protein disulfide isomerase P5; Thioredoxin domain-containing protein 7 

Gene Name

 PDIA6 

Gene Synonyms/Alias

 ERP5; P5; TXNDC7 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
154	VQGFPTIKIFGSNKN	ubiquitination	[1]
160	IKIFGSNKNRPEDYQ	ubiquitination	[1, 2]
268	EQTKGKVKLAAVDAT	ubiquitination	[3]
293	IRGFPTIKIFQKGES	ubiquitination	[1, 2, 3]
297	PTIKIFQKGESPVDY	ubiquitination	[2, 3]
420	AINARKMKFALLKGS	ubiquitination	[2]

Reference

 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin. 

Sequence Annotation

 DOMAIN 20 133 Thioredoxin 1.
 DOMAIN 154 287 Thioredoxin 2.
 MOTIF 437 440 Prevents secretion from ER (Potential).
 ACT_SITE 55 55 Nucleophile (By similarity).
 ACT_SITE 58 58 Nucleophile (By similarity).
 ACT_SITE 190 190 Nucleophile (By similarity).
 ACT_SITE 193 193 Nucleophile (By similarity).
 MOD_RES 428 428 Phosphoserine.
 DISULFID 55 58 Redox-active (By similarity).
 DISULFID 190 193 Redox-active (By similarity).  

Keyword

 3D-structure; Alternative splicing; Cell membrane; Chaperone; Complete proteome; Direct protein sequencing; Disulfide bond; Endoplasmic reticulum; Isomerase; Membrane; Phosphoprotein; Polymorphism; Redox-active center; Reference proteome; Repeat; Signal. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 440 AA 

Protein Sequence

Gene Ontology

 GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IDA:UniProtKB.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0003756; F:protein disulfide isomerase activity; TAS:ProtInc.
 GO:0015035; F:protein disulfide oxidoreductase activity; IEA:InterPro.
 GO:0006987; P:activation of signaling protein activity involved in unfolded protein response; TAS:Reactome.
 GO:0045454; P:cell redox homeostasis; IEA:InterPro.
 GO:0006662; P:glycerol ether metabolic process; IEA:InterPro. 

Interpro

 IPR005788; Disulphide_isomerase.
 IPR005746; Thioredoxin.
 IPR012336; Thioredoxin-like_fold.
 IPR017937; Thioredoxin_CS.
 IPR013766; Thioredoxin_domain. 

Pfam

 PF00085; Thioredoxin 

SMART

  

PROSITE

 PS00014; ER_TARGET
 PS00194; THIOREDOXIN_1
 PS51352; THIOREDOXIN_2 

PRINTS

 PR00421; THIOREDOXIN.