CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011286
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Hormone-sensitive lipase 
Protein Synonyms/Alias
 HSL 
Gene Name
 LIPE 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
624EELSSLIKSNGQRSLubiquitination[1, 2, 3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. 
Sequence Annotation
 ACT_SITE 651 651 Potential.
 ACT_SITE 725 725 Potential.
 MOD_RES 853 853 Phosphoserine (By similarity).
 MOD_RES 855 855 Phosphoserine; by AMPK (By similarity).
 MOD_RES 950 950 Phosphoserine (By similarity).
 MOD_RES 951 951 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Cell membrane; Cholesterol metabolism; Complete proteome; Cytoplasm; Hydrolase; Lipid degradation; Lipid metabolism; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Steroid metabolism; Sterol metabolism. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1076 AA 
Protein Sequence
MEPGSKSVSR SDWQPEPHQR PITPLEPGPE KTPIAQPESK TLQGSNTQQK PASNQRPLTQ 60
QETPAQHDAE SQKEPRAQQK SASQEEFLAP QKPAPQQSPY IQRVLLTQQE AASQQGPGLG 120
KESITQQEPA LRQRHVAQPG PGPGEPPPAQ QEAESTPAAQ AKPGAKREPS APTESTSQET 180
PEQSDKQTTP VQGAKSKQGS LTELGFLTKL QELSIQRSAL EWKALSEWVT DSESESDVGS 240
SSDTDSPATM GGMVAQGVKL GFKGKSGYKV MSGYSGTSPH EKTSARNHRH YQDTASRLIH 300
NMDLRTMTQS LVTLAEDNIA FFSSQGPGET AQRLSGVFAG VREQALGLEP ALGRLLGVAH 360
LFDLDPETPA NGYRSLVHTA RCCLAHLLHK SRYVASNRRS IFFRTSHNLA ELEAYLAALT 420
QLRALVYYAQ RLLVTNRPGV LFFEGDEGLT ADFLREYVTL HKGCFYGRCL GFQFTPAIRP 480
FLQTISIGLV SFGEHYKRNE TGLSVAASSL FTSGRFAIDP ELRGAEFERI TQNLDVHFWK 540
AFWNITEMEV LSSLANMASA TVRVSRLLSL PPEAFEMPLT ADPTLTVTIS PPLAHTGPGP 600
VLVRLISYDL REGQDSEELS SLIKSNGQRS LELWPRPQQA PRSRSLIVHF HGGGFVAQTS 660
RSHEPYLKSW AQELGAPIIS IDYSLAPEAP FPRALEECFF AYCWAIKHCA LLGSTGERIC 720
LAGDSAGGNL CFTVALRAAA YGVRVPDGIM AAYPATMLQP AASPSRLLSL MDPLLPLSVL 780
SKCVSAYAGA KTEDHSNSDQ KALGMMGLVR RDTALLLRDF RLGASSWLNS FLELSGRKSQ 840
KMSEPIAEPM RRSVSEAALA QPQGPLGTDS LKNLTLRDLS LRGNSETSSD TPEMSLSAET 900
LSPSTPSDVN FLLPPEDAGE EAEAKNELSP MDRGLGVRAA FPEGFHPRRS SQGATQMPLY 960
SSPIVKNPFM SPLLAPDSML KSLPPVHIVA CALDPMLDDS VMLARRLRNL GQPVTLRVVE 1020
DLPHGFLTLA ALCRETRQAA ELCVERIRLV LTPPAGAGPS GETGAAGVDG GCGGRH 1076 
Gene Ontology
 GO:0005901; C:caveola; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005811; C:lipid particle; ISS:UniProtKB.
 GO:0033878; F:hormone-sensitive lipase activity; IEA:EC.
 GO:0004806; F:triglyceride lipase activity; IEA:Compara.
 GO:0008203; P:cholesterol metabolic process; IEA:UniProtKB-KW.
 GO:0046340; P:diacylglycerol catabolic process; IEA:Compara.
 GO:0006468; P:protein phosphorylation; TAS:ProtInc.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0019433; P:triglyceride catabolic process; TAS:Reactome. 
Interpro
 IPR013094; AB_hydrolase_3.
 IPR010468; HSL_N.
 IPR002168; Lipase_GDXG_AS. 
Pfam
 PF07859; Abhydrolase_3
 PF06350; HSL_N 
SMART
  
PROSITE
 PS01173; LIPASE_GDXG_HIS
 PS01174; LIPASE_GDXG_SER 
PRINTS