UniProt Accession

 SPT20_YEAST; P50875; D6W1S1 

Genbank Protein ID

 U43153; U22063; Z74890; Z48239; BK006948 

Genbank Nucleotide ID

 AAB07899.1; AAB07900.1; CAA99169.1; CAA88279.1; DAA10637.1 

Protein Name

 Transcription factor SPT20 

Protein Synonyms/Alias

  

Gene Name

 SPT20 

Gene Synonyms/Alias

 ADA5; YOL148C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
570	PKVKRPRKNAKKSES	acetylation	[1]
573	KRPRKNAKKSESGTP	acetylation	[1]
574	RPRKNAKKSESGTPA	acetylation	[1]

Reference

 [1] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591] 

Functional Description

 Transcription regulator. May recruit TATA binding protein (TBP) and possibly other basal factors to bind to the TATA box. Functions as component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus. 

Sequence Annotation

 MOD_RES 446 446 Phosphoserine.
 MOD_RES 516 516 Phosphothreonine.  

Keyword

 Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 604 AA 

Protein Sequence

Gene Ontology

 GO:0000124; C:SAGA complex; IDA:SGD.
 GO:0046695; C:SLIK (SAGA-like) complex; IDA:SGD.
 GO:0003712; F:transcription cofactor activity; IPI:SGD.
 GO:0016573; P:histone acetylation; IDA:SGD.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0030969; P:UFP-specific transcription factor mRNA processing involved in endoplasmic reticulum unfolded protein response; IMP:SGD. 

Interpro

 IPR021950; Spt20. 

Pfam

 PF12090; Spt20 

SMART

  

PROSITE

  

PRINTS