UniProt Accession

 TAF12_YEAST; Q03761; D6VSC8 

Genbank Protein ID

 Z50046; BK006938 

Genbank Nucleotide ID

 CAA90368.1; DAA11988.1 

Protein Name

 Transcription initiation factor TFIID subunit 12 

Protein Synonyms/Alias

 TAFII-61; TAFII61; TAFII-68; TAFII68; TBP-associated factor 12; TBP-associated factor 61 kDa 

Gene Name

 TAF12 

Gene Synonyms/Alias

 TAF61; TAF68; YDR145W; YD8358.02 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
131	QTRNNSNKFSNMIKQ	acetylation	[1]
351	LNNISSSKSAIFKQT	acetylation	[1]
356	SSKSAIFKQTEPAIP	acetylation	[1]
371	ISENISTKTPAPVAY	acetylation	[1]
526	SDKVAAAKNNGNNVA	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Functions as a component of the DNA-binding general transcription factor complex TFIID and the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus. 

Sequence Annotation

 DOMAIN 413 490 Histone-fold.
 MOD_RES 129 129 Phosphoserine.
 MOD_RES 286 286 Phosphoserine.  

Keyword

 Coiled coil; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 539 AA 

Protein Sequence

Gene Ontology

 GO:0000124; C:SAGA complex; IDA:SGD.
 GO:0046695; C:SLIK (SAGA-like) complex; IDA:SGD.
 GO:0005669; C:transcription factor TFIID complex; IDA:SGD.
 GO:0003682; F:chromatin binding; IDA:SGD.
 GO:0032947; F:protein complex scaffold; IMP:SGD.
 GO:0001102; F:RNA polymerase II activating transcription factor binding; IDA:SGD.
 GO:0017025; F:TBP-class protein binding; IDA:SGD.
 GO:0016573; P:histone acetylation; IDA:SGD.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0051123; P:RNA polymerase II transcriptional preinitiation complex assembly; IMP:SGD. 

Interpro

 IPR009072; Histone-fold.
 IPR003228; TFIID_sub. 

Pfam

 PF03847; TFIID_20kDa 

SMART

  

PROSITE

  

PRINTS