CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031536
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 FERM domain-containing protein 8 
Protein Synonyms/Alias
 cDNA FLJ52403, highly similar to Homo sapiens FKSG44 gene (FKSG44), mRNA 
Gene Name
 FRMD8 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
99RRNVFFPKRRELQIHubiquitination[1, 2]
119RLLYEEAKGNVLAARubiquitination[1, 2, 3, 4, 5]
162AACDLREKLDSFLPAubiquitination[1, 2]
173FLPAHLCKRGQSLFAubiquitination[1, 2]
226HYRAYLLKCHELPFYubiquitination[1]
275HVIDSREKHVLLGLRubiquitination[1]
318TPVNKLLKIYSKQAEubiquitination[1]
393IDYVEDGKGIRRVKPubiquitination[1, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 430 AA 
Protein Sequence
MDGTEGSAGQ PGPAERSHRS SVSSVGARAA DVLVYLADDT VVPLAVENLP SLSAHELHRA 60
VREVLQLPDI ALDVFALWLV SPLLDEPFLQ FRRNVFFPKR RELQIHDEEV LRLLYEEAKG 120
NVLAARYPCD VEDCEALGAL VCRVQLGPYQ PGRPAACDLR EKLDSFLPAH LCKRGQSLFA 180
ALRGRGARAG PGEQGLLNAY RQVQEVSSDG GCEAALGTHY RAYLLKCHEL PFYGCAFFHG 240
EVDKPAQGFL HRGGRKPVSV AISLEGVHVI DSREKHVLLG LRFQELSWDH TSPEEEEPIL 300
WLEFDGDSEG TPVNKLLKIY SKQAELMSSL IEYCIELSQA AEPAGPQDSA TGSPSDPSSS 360
LAPVQRPKLR RQGSVVSSRI QHLSTIDYVE DGKGIRRVKP KRTTSFFSRQ LSLGQGSYTV 420
VQPGDSLEQG 430 
Gene Ontology
 GO:0005856; C:cytoskeleton; IEA:InterPro. 
Interpro
 IPR019749; Band_41_domain.
 IPR014352; FERM/acyl-CoA-bd_prot_3-hlx.
 IPR019748; FERM_central.
 IPR000299; FERM_domain. 
Pfam
 PF00373; FERM_M 
SMART
 SM00295; B41 
PROSITE
 PS50057; FERM_3 
PRINTS