CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-043705
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Aspartate--tRNA ligase, cytoplasmic 
Protein Synonyms/Alias
  
Gene Name
 DARS 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
44TEIQTVNKQFPCEPFubiquitination[1, 2, 3]
52QFPCEPFKFLEPTLRubiquitination[2, 3]
88DLSTPNEKLLGHLVKacetylation[4]
88DLSTPNEKLLGHLVKubiquitination[2, 5]
138HHGIDLEKIKAYIDSacetylation[3, 4]
138HHGIDLEKIKAYIDSubiquitination[2]
140GIDLEKIKAYIDSFRubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
  
Sequence Annotation
  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 188 AA 
Protein Sequence
VGLDIEMAFN YHYHEVMEEI ADTMVQIFKG LQERFQTEIQ TVNKQFPCEP FKFLEPTLRL 60
EYCEALAMLR EAGVEMGDED DLSTPNEKLL GHLVKEKKQS NSYDMFMRGE EILSGAQRIH 120
DPQLLTERAL HHGIDLEKIK AYIDSFRFGA PPHAGGGIGL ERVTMLFLGL HNVRQTSMFP 180
RDPKRLTP 188 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0004815; F:aspartate-tRNA ligase activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0006422; P:aspartyl-tRNA aminoacylation; IEA:InterPro. 
Interpro
 IPR004364; aa-tRNA-synt_II.
 IPR018150; aa-tRNA-synt_II-like.
 IPR006195; aa-tRNA-synth_II.
 IPR004523; Asp-tRNA_synthase.
 IPR002312; Asp/Asn-tRNA-synth_IIb. 
Pfam
 PF00152; tRNA-synt_2 
SMART
  
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS
 PR01042; TRNASYNTHASP.