CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021087
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase/endoribonuclease IRE1 
Protein Synonyms/Alias
 Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1; Ire1-alpha; IRE1a; Serine/threonine-protein kinase; Endoribonuclease 
Gene Name
 Ern1 
Gene Synonyms/Alias
 Ire1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
706PNAHGRIKAMISDFGubiquitination[1]
811IARELIEKMIAMDPQubiquitination[1]
871RGGRAVVKMDWRENIubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto- activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. 
Sequence Annotation
 DOMAIN 571 832 Protein kinase.
 DOMAIN 835 963 KEN.
 NP_BIND 577 585 ATP (By similarity).
 ACT_SITE 688 688 Proton acceptor (By similarity).
 BINDING 599 599 ATP (By similarity).
 CARBOHYD 178 178 N-linked (GlcNAc...) (Potential).  
Keyword
 ADP-ribosylation; Alternative splicing; Apoptosis; ATP-binding; Complete proteome; Disulfide bond; Endoplasmic reticulum; Glycoprotein; Hydrolase; Kinase; Magnesium; Membrane; Metal-binding; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Signal; Transcription; Transcription regulation; Transferase; Transmembrane; Transmembrane helix; Unfolded protein response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 977 AA 
Protein Sequence
MPARWLLLLL ALLLPPPGPG SFGRTSTVTL PETLLFVSTL DGSLHAVSKR TGSIKWTLKE 60
DPVLQVPTHV EEPAFLPDPN DGSLYTLGGK NNEGLTKLPF TIPELVQASP CRSSDGILYM 120
GKKQDIWYVI DLLTGEKQQT LSSAFADSLC PSTSLLYLGR TEYTITMYDT KTRELRWNAT 180
YFDYAASLPE DDVDYKMSHF VSNGDGLVVT VDSESGDVLW IQNYASPVVA FYVWQGEVLR 240
KVVHINVAVE TLRYLTFMSG EVGRITKWKY PFPKETEAKS KLTPTLYVGK YSTSLYASPS 300
MVHEGVAVVP RGSTLPLLEG PQTDGVTIGD KGECVITPST DLKFDPGLKG KSKLNYLRNY 360
WLLIGHHETP LSASTKMLER FPNNLPKHRE NVIPADSEKR SFEEVINIVG QTSDNTPTTV 420
SQDVEEKLAR APAKPEAPVD SMLKDMATII LSTFLLVGWV AFIITYPLSV HQQRQLQHQQ 480
FQKELEKIQL LQQQQLPFHP HGDLTQDPEF LDSSGPFSES SGTSSPSPSP RASNHSLHPS 540
SSASRAGTSP SLEQDDEDEE TRMVIVGKIS FCPKDVLGHG AEGTIVYKGM FDNRDVAVKR 600
ILPECFSFAD REVQLLRESD EHPNVIRYFC TEKDRQFQYI AIELCAATLQ EYVEQKDFAH 660
LGLEPITLLH QTTSGLAHLH SLNIVHRDLK PHNILLSMPN AHGRIKAMIS DFGLCKKLAV 720
GRHSFSRRSG VPGTEGWIAP EMLSEDCKDN PTYTVDIFSA GCVFYYVISE GNHPFGKSLQ 780
RQANILLGAC NLDCFHSDKH EDVIARELIE KMIAMDPQQR PSAKHVLKHP FFWSLEKQLQ 840
FFQDVSDRIE KEALDGPIVR QLERGGRAVV KMDWRENITV PLQTDLRKFR TYKGGSVRDL 900
LRAMRNKKHH YRELPVEVQE TLGSIPDDFV RYFTSRFPHL LSHTYQAMEL CRHERLFQTY 960
YWHEPTEPQP PVIPYAL 977 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IDA:MGI.
 GO:0030176; C:integral to endoplasmic reticulum membrane; IEA:Compara.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005637; C:nuclear inner membrane; IDA:UniProtKB.
 GO:0005524; F:ATP binding; ISS:UniProtKB.
 GO:0004521; F:endoribonuclease activity; IDA:MGI.
 GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; ISS:UniProtKB.
 GO:0006987; P:activation of signaling protein activity involved in unfolded protein response; ISS:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0007050; P:cell cycle arrest; IDA:UniProtKB.
 GO:0006917; P:induction of apoptosis; IDA:UniProtKB.
 GO:0006397; P:mRNA processing; IEA:InterPro.
 GO:1900103; P:positive regulation of endoplasmic reticulum unfolded protein response; IEA:Compara.
 GO:0046777; P:protein autophosphorylation; IDA:MGI.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR010513; KEN_dom.
 IPR011009; Kinase-like_dom.
 IPR018391; PQQ_beta_propeller_repeat.
 IPR000719; Prot_kinase_cat_dom.
 IPR006567; PUG-dom.
 IPR027295; Quinonprotein_ADH-like_fam.
 IPR011047; Quinonprotein_ADH-like_supfam.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase
 PF06479; Ribonuc_2-5A 
SMART
 SM00564; PQQ
 SM00580; PUG 
PROSITE
 PS51392; KEN
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS