CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037960
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Beta-adrenergic receptor kinase 1 
Protein Synonyms/Alias
  
Gene Name
 ADRBK1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
31PAARASKKILLPEPSubiquitination[1]
45SIRSVMQKYLEDRGEubiquitination[1]
57RGEVTFEKIFSQKLGubiquitination[1, 2]
62FEKIFSQKLGYLLFRubiquitination[1, 2, 3]
94EEIKKYEKLETEEERubiquitination[1]
115IFDSYIMKELLACSHubiquitination[1]
126ACSHPFSKSATEHVQubiquitination[1, 3]
139VQGHLGKKQVPPDLFubiquitination[1]
164LRGDVFQKFIESDKFubiquitination[1]
170QKFIESDKFTRFCQWubiquitination[1, 2]
215CRKADTGKMYAMKCLubiquitination[1]
220TGKMYAMKCLDKKRIubiquitination[1, 4]
230DKKRIKMKQGETLALubiquitination[1, 3]
319FVVYRDLKPANILLDubiquitination[1, 3]
344GLACDFSKKKPHASVubiquitination[1, 3, 4]
345LACDFSKKKPHASVGubiquitination[1, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 408 AA 
Protein Sequence
MADLEAVLAD VSYLMAMEKS KATPAARASK KILLPEPSIR SVMQKYLEDR GEVTFEKIFS 60
QKLGYLLFRD FCLNHLEEAR PLVEFYEEIK KYEKLETEEE RVARSREIFD SYIMKELLAC 120
SHPFSKSATE HVQGHLGKKQ VPPDLFQPYI EEICQNLRGD VFQKFIESDK FTRFCQWKNV 180
ELNIHLTMND FSVHRIIGRG GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER 240
IMLSLVSTGD CPFIVCMSYA FHTPDKLSFI LDLMNGGDLH YHLSQHGVFS EADMRFYAAE 300
IILGLEHMHN RFVVYRDLKP ANILLDEHGH VRISDLGLAC DFSKKKPHAS VGTHGYMAPE 360
VLQKGSRCLC GVAPSPPRGW VGAPSPPVYS FPGAFLCRFL NVILKSEK 408 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004703; F:G-protein coupled receptor kinase activity; IEA:InterPro.
 GO:0007165; P:signal transduction; IEA:InterPro.
 GO:0038032; P:termination of G-protein coupled receptor signaling pathway; IEA:InterPro. 
Interpro
 IPR000239; GPCR_kinase.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR000342; Regulat_G_prot_signal.
 IPR016137; Regulat_G_prot_signal_superfam.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase
 PF00615; RGS 
SMART
 SM00315; RGS 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS50132; RGS 
PRINTS
 PR00717; GPCRKINASE.