CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032903
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Transitional endoplasmic reticulum ATPase 
Protein Synonyms/Alias
  
Gene Name
 VCP 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
15RGDTVLLKGKKRREAubiquitination[1, 2, 3, 4, 5]
18TVLLKGKKRREAVCIubiquitination[3]
36DDTCSDEKIRMNRVVubiquitination[3, 5, 6]
64IQPCPDVKYGKRIHVubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
67CPDVKYGKRIHVLPIubiquitination[1, 2, 3, 5]
103EAYRPIRKGDIFLVRubiquitination[2, 3, 5, 6]
119GMRAVEFKVVETDPSubiquitination[1, 3, 6]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 160 AA 
Protein Sequence
MDELQLFRGD TVLLKGKKRR EAVCIVLSDD TCSDEKIRMN RVVRNNLRVR LGDVISIQPC 60
PDVKYGKRIH VLPIDDTVEG ITGNLFEVYL KPYFLEAYRP IRKGDIFLVR GGMRAVEFKV 120
VETDPSPYCI VAPDTVIHCE GEPIKREDEE ESLNEVGYDD 160 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005783; C:endoplasmic reticulum; IEA:Compara.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:Compara.
 GO:0016887; F:ATPase activity; IEA:Compara.
 GO:0006919; P:activation of cysteine-type endopeptidase activity involved in apoptotic process; IEA:Compara.
 GO:0070842; P:aggresome assembly; IEA:Compara.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; IEA:Compara.
 GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; IEA:Compara.
 GO:0051260; P:protein homooligomerization; IEA:Compara.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:Compara. 
Interpro
 IPR009010; Asp_de-COase-like_dom.
 IPR005938; ATPase_AAA_CDC48.
 IPR004201; Cdc48_dom2.
 IPR003338; CDC4_N-term_subdom. 
Pfam
 PF02933; CDC48_2
 PF02359; CDC48_N 
SMART
 SM01072; CDC48_2
 SM01073; CDC48_N 
PROSITE
  
PRINTS