CPLM-002435

Genbank Nucleotide ID

Acyl-CoA-binding protein

Protein Synonyms/Alias

ACBP; Diazepam-binding inhibitor; DBI; Endozepine; EP

Homo sapiens (Human)

Position	Peptide	Type	References
8	MSQAEFEKAAEEVRH	acetylation	[1]
17	AEEVRHLKTKPSDEE	acetylation	[1, 2]
19	EVRHLKTKPSDEEML	acetylation	[1, 2, 3, 4]
53	GMLDFTGKAKWDAWN	acetylation	[3]
53	GMLDFTGKAKWDAWN	ubiquitination	[5, 6]
55	LDFTGKAKWDAWNEL	acetylation	[1, 2, 4]
55	LDFTGKAKWDAWNEL	malonylation	[7]
55	LDFTGKAKWDAWNEL	ubiquitination	[5, 8]
63	WDAWNELKGTSKEDA	ubiquitination	[5]
72	TSKEDAMKAYINKVE	acetylation	[9]
77	AMKAYINKVEELKKK	acetylation	[1, 2, 10]
77	AMKAYINKVEELKKK	ubiquitination	[5, 6, 11]

[1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[2] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
[3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
[7] The first identification of lysine malonylation substrates and its regulatory enzyme.
Peng C, Lu Z, Xie Z, Cheng Z, Chen Y, Tan M, Luo H, Zhang Y, He W, Yang K, Zwaans BM, Tishkoff D, Ho L, Lombard D, He TC, Dai J, Verdin E, Ye Y, Zhao Y.
Mol Cell Proteomics. 2011 Dec;10(12):M111.012658. [PMID: 21908771]
[8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[9] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
[10] Regulation of cellular metabolism by protein lysine acetylation.
Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
[11] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]

Functional Description

Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.

DOMAIN 2 87 ACB.
REGION 29 33 Acyl-CoA binding.
BINDING 14 14 Acyl-CoA.
BINDING 55 55 Acyl-CoA.
BINDING 74 74 Acyl-CoA.
MOD_RES 2 2 N-acetylserine.
MOD_RES 8 8 N6-acetyllysine.
MOD_RES 19 19 N6-acetyllysine.
MOD_RES 29 29 Phosphotyrosine.
MOD_RES 55 55 N6-acetyllysine; alternate.
MOD_RES 55 55 N6-malonyllysine; alternate.
MOD_RES 77 77 N6-acetyllysine.

3D-structure; Acetylation; Alternative promoter usage; Alternative splicing; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Golgi apparatus; Lipid-binding; Phosphoprotein; Polymorphism; Reference proteome; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
GO:0005739; C:mitochondrion; IEA:Compara.
GO:0030156; F:benzodiazepine receptor binding; TAS:ProtInc.
GO:0000062; F:fatty-acyl-CoA binding; IEA:InterPro.
GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
GO:0001942; P:hair follicle development; IEA:Compara.
GO:0043588; P:skin development; IEA:Compara.
GO:0006810; P:transport; IEA:UniProtKB-KW.
GO:0006641; P:triglyceride metabolic process; IEA:Compara.

IPR022408; Acyl-CoA-binding_prot_CS.
IPR000582; Acyl-CoA-binding_protein.
IPR014352; FERM/acyl-CoA-bd_prot_3-hlx.

PS00880; ACB_1
PS51228; ACB_2

PR00689; ACOABINDINGP.