CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001337
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Heat stress transcription factor A-2 
Protein Synonyms/Alias
 AtHsfA2; AtHsf-04 
Gene Name
 HSFA2 
Gene Synonyms/Alias
 HSF04; At2g26150; T19L18.4 
Created Date
 July 27, 2013 
Organism
 Arabidopsis thaliana (Mouse-ear cress) 
NCBI Taxa ID
 3702 
Lysine Modification
Position
Peptide
Type
References
167DGEVERLKRDHGVLVsumoylation[1]
315LEAALDVKVEDLVGSsumoylation[1]
Reference
 [1] Sumoylation of Arabidopsis heat shock factor A2 (HsfA2) modifies its activity during acquired thermotholerance.
 Cohen-Peer R, Schuster S, Meiri D, Breiman A, Avni A.
 Plant Mol Biol. 2010 Sep;74(1-2):33-45. [PMID: 20521085
Functional Description
 Transcriptional activator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE). Involved in heat stress responses. Seems to be involved in other environmental stress responses. Activates ascorbate peroxidase 2 (APX2) in addition to several heat shock protein (HSPs). 
Sequence Annotation
 DNA_BIND 42 136 By similarity.
 REGION 150 216 Hydrophobic repeat HR-A/B.
 MOTIF 230 245 Bipartite nuclear localization signal
 MOTIF 273 282 AHA1.
 MOTIF 324 333 AHA2.
 MOTIF 334 341 Nuclear export signal (Potential).
 CROSSLNK 315 315 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Activator; Alternative splicing; Complete proteome; Cytoplasm; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Repeat; Stress response; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 345 AA 
Protein Sequence
MEELKVEMEE ETVTFTGSVA ASSSVGSSSS PRPMEGLNET GPPPFLTKTY EMVEDPATDT 60
VVSWSNGRNS FVVWDSHKFS TTLLPRYFKH SNFSSFIRQL NTYGFRKIDP DRWEFANEGF 120
LAGQKHLLKN IKRRRNMGLQ NVNQQGSGMS CVEVGQYGFD GEVERLKRDH GVLVAEVVRL 180
RQQQHSSKSQ VAAMEQRLLV TEKRQQQMMT FLAKALNNPN FVQQFAVMSK EKKSLFGLDV 240
GRKRRLTSTP SLGTMEENLL HDQEFDRMKD DMEMLFAAAI DDEANNSMPT KEEQCLEAMN 300
VMMRDGNLEA ALDVKVEDLV GSPLDWDSQD LHDMVDQMGF LGSEP 345 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; ISS:TAIR.
 GO:0034605; P:cellular response to heat; IEP:TAIR.
 GO:0071456; P:cellular response to hypoxia; IEP:TAIR.
 GO:0034620; P:cellular response to unfolded protein; IEP:TAIR.
 GO:0010286; P:heat acclimation; IMP:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0010200; P:response to chitin; IEP:TAIR.
 GO:0009644; P:response to high light intensity; IEP:TAIR.
 GO:0042542; P:response to hydrogen peroxide; IEP:TAIR.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR000232; HSF_DNA-bd.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00447; HSF_DNA-bind 
SMART
 SM00415; HSF 
PROSITE
 PS00434; HSF_DOMAIN 
PRINTS
 PR00056; HSFDOMAIN.