UniProt Accession

 CISY_ECOLI; P0ABH7; O32552; P00891; P78257 

Genbank Protein ID

 J01619; U00096; AP009048; X00980; V01501; M28987; M29373 

Genbank Nucleotide ID

 AAA23892.1; AAC73814.1; BAA35384.1; CAA25484.1; CAA24746.1; AAA23901.1; AAA23902.1 

Protein Name

 Citrate synthase 

Protein Synonyms/Alias

  

Gene Name

 gltA 

Gene Synonyms/Alias

 gluT; icdB; b0720; JW0710 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
7	*MADTKAKLTLNGDT	acetylation	[1]
38	DIRTLGSKGVFTFDP	acetylation	[1, 2]
56	STASCESKITFIDGD	acetylation	[2]
105	QEQYDEFKTTVTRHT	acetylation	[1]
168	AAFRLLSKMPTMAAM	acetylation	[1]
283	LEEISSVKHIPEFVR	acetylation	[1, 2, 3, 4]
295	FVRRAKDKNDSFRLM	acetylation	[1, 2]
310	GFGHRVYKNYDPRAT	acetylation	[1, 2]
328	ETCHEVLKELGTKDD	acetylation	[1]
356	NDPYFIEKKLYPNVD	acetylation	[2, 4]
405	EMHSDGMKIARPRQL	acetylation	[1, 2]
418	QLYTGYEKRDFKSDI	acetylation	[1]
422	GYEKRDFKSDIKR**	acetylation	[1, 2]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [3] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842]
 [4] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508] 

Functional Description

  

Sequence Annotation

 ACT_SITE 306 306 By similarity.
 ACT_SITE 363 363 By similarity.
 MOD_RES 283 283 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Allosteric enzyme; Complete proteome; Direct protein sequencing; Reference proteome; Transferase; Tricarboxylic acid cycle. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 427 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0004108; F:citrate (Si)-synthase activity; IEA:EC.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway. 

Interpro

 IPR016142; Citrate_synth-like_lrg_a-sub.
 IPR016143; Citrate_synth-like_sm_a-sub.
 IPR002020; Citrate_synthase-like.
 IPR016141; Citrate_synthase-like_core.
 IPR019810; Citrate_synthase_AS.
 IPR024176; Citrate_synthase_bac-typ.
 IPR010953; Citrate_synthase_typ-II. 

Pfam

 PF00285; Citrate_synt 

SMART

  

PROSITE

 PS00480; CITRATE_SYNTHASE 

PRINTS

 PR00143; CITRTSNTHASE.