CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022511
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 GEM-interacting protein 
Protein Synonyms/Alias
 GMIP 
Gene Name
 GMIP 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
91DLRLIRTKGGVDAALubiquitination[1]
117ELLAWTEKRASYELEubiquitination[1]
184YYQPLAAKRTEIEKWubiquitination[1, 2]
196EKWRKEFKEQWMKEQubiquitination[1]
248QASPGPSKQQERRRRubiquitination[1]
289QQDLEIAKQRIVSHVubiquitination[1]
624HDVSSVLKRFLQELTubiquitination[3, 4]
672PEVIRSLKTLLVQLPubiquitination[3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Stimulates, in vitro and in vivo, the GTPase activity of RhoA. 
Sequence Annotation
 DOMAIN 554 757 Rho-GAP.
 ZN_FING 493 537 Phorbol-ester/DAG-type.
 MOD_RES 71 71 Phosphoserine (By similarity).
 MOD_RES 234 234 Phosphoserine.
 MOD_RES 243 243 Phosphoserine.
 MOD_RES 437 437 Phosphoserine (By similarity).  
Keyword
 3D-structure; Complete proteome; GTPase activation; Metal-binding; Phosphoprotein; Polymorphism; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 970 AA 
Protein Sequence
MDAAEPGLPP GPEGRKRYSD IFRSLDNLEI SLGNVTLEML AGDPLLSEDP EPDKTPTATV 60
TNEASCWSGP SPEGPVPLTG EELDLRLIRT KGGVDAALEY AKTWSRYAKE LLAWTEKRAS 120
YELEFAKSTM KIAEAGKVSI QQQSHMPLQY IYTLFLEHDL SLGTLAMETV AQQKRDYYQP 180
LAAKRTEIEK WRKEFKEQWM KEQKRMNEAV QALRRAQLQY VQRSEDLRAR SQGSPEDSAP 240
QASPGPSKQQ ERRRRSREEA QAKAQEAEAL YQACVREANA RQQDLEIAKQ RIVSHVRKLV 300
FQGDEVLRRV TLSLFGLRGA QAERGPRAFA ALAECCAPFE PGQRYQEFVR ALRPEAPPPP 360
PPAFSFQEFL PSLNSSPLDI RKKLSGPLPP RLDENSAEPG PWEDPGTGWR WQGTPGPTPG 420
SDVDSVGGGS ESRSLDSPTS SPGAGTRQLV KASSTGTESS DDFEERDPDL GDGLENGLGS 480
PFGKWTLSSA AQTHQLRRLR GPAKCRECEA FMVSGTECEE CFLTCHKRCL ETLLILCGHR 540
RLPARTPLFG VDFLQLPRDF PEEVPFVVTK CTAEIEHRAL DVQGIYRVSG SRVRVERLCQ 600
AFENGRALVE LSGNSPHDVS SVLKRFLQEL TEPVIPFHLY DAFISLAKTL HADPGDDPGT 660
PSPSPEVIRS LKTLLVQLPD SNYNTLRHLV AHLFRVAARF MENKMSANNL GIVFGPTLLR 720
PPDGPRAASA IPVTCLLDSG HQAQLVEFLI VHYEQIFGMD ELPQATEPPP QDSSPAPGPL 780
TTSSQPPPPH LDPDSQPPVL ASDPGPDPQH HSTLEQHPTA TPTEIPTPQS DQREDVAEDT 840
KDGGGEVSSQ GPEDSLLGTQ SRGHFSRQPV KYPRGGVRPV THQLSSLALV ASKLCEETPI 900
TSVPRGSLRG RGPSPAAASP EGSPLRRTPL PKHFEITQET ARLLSKLDSE AVPRATCCPD 960
VQPEEAEDHL 970 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005100; F:Rho GTPase activator activity; IDA:HGNC.
 GO:0034259; P:negative regulation of Rho GTPase activity; IDA:BHF-UCL.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome. 
Interpro
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom. 
Pfam
 PF00620; RhoGAP 
SMART
 SM00109; C1
 SM00324; RhoGAP 
PROSITE
 PS50238; RHOGAP
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS