UniProt Accession

 G3BP1_MOUSE; P97855 

Genbank Protein ID

 AB001927; BC021156 

Genbank Nucleotide ID

 BAA19469.1; AAH21156.1 

Protein Name

 Ras GTPase-activating protein-binding protein 1 

Protein Synonyms/Alias

 G3BP-1; ATP-dependent DNA helicase VIII; GAP SH3 domain-binding protein 1; HDH-VIII 

Gene Name

 G3bp1 

Gene Synonyms/Alias

 G3bp 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
5	***MVMEKPSPLLVG	ubiquitination	[1]
50	GGLDSNGKPADAVYG	ubiquitination	[1]
76	NFTNCHTKIRHVDAH	acetylation	[2]
123	PEGSVANKFYVHNDI	ubiquitination	[1]
351	NLPHEVDKSELKDFF	acetylation	[3]
351	NLPHEVDKSELKDFF	ubiquitination	[1]
374	LRINSGGKLPNFGFV	acetylation	[4]
374	LRINSGGKLPNFGFV	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium- dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. 

Sequence Annotation

 DOMAIN 11 133 NTF2.
 DOMAIN 338 413 RRM.
 MOD_RES 143 143 Phosphothreonine (By similarity).
 MOD_RES 149 149 Phosphoserine.
 MOD_RES 231 231 Phosphoserine.
 MOD_RES 266 266 Phosphothreonine.
 MOD_RES 371 371 Phosphoserine (By similarity).
 MOD_RES 374 374 N6-acetyllysine (By similarity).
 MOD_RES 433 433 Dimethylated arginine; alternate (By
 MOD_RES 433 433 Omega-N-methylarginine; alternate (By
 MOD_RES 445 445 Omega-N-methylated arginine (By
 MOD_RES 458 458 Dimethylated arginine; alternate (By
 MOD_RES 458 458 Omega-N-methylated arginine; alternate
 MOD_RES 464 464 Omega-N-methylated arginine (By  

Keyword

 Acetylation; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Endonuclease; Helicase; Hydrolase; Membrane; Methylation; Nuclease; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 465 AA 

Protein Sequence

Gene Ontology

 GO:0010494; C:cytoplasmic stress granule; IDA:MGI.
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
 GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
 GO:0003729; F:mRNA binding; IDA:MGI.
 GO:0090090; P:negative regulation of canonical Wnt receptor signaling pathway; IMP:MGI.
 GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC.
 GO:0006810; P:transport; IEA:UniProtKB-KW. 

Interpro

 IPR002075; NTF2.
 IPR018222; Nuclear_transport_factor_2_euk.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 

Pfam

 PF02136; NTF2
 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50177; NTF2_DOMAIN
 PS50102; RRM 

PRINTS