UniProt Accession

 RPB11_HUMAN; P52435; A5D6V8; O43375 

Genbank Protein ID

 X82385; L37127; X98433; AK290515; AC093668; BC024165; BC065711; BC139902; BC141830 

Genbank Nucleotide ID

 CAA57785.1; AAD05361.1; CAA67075.1; BAF83204.1; AAH24165.1; AAH65711.1; AAI39903.1; AAI41831.1 

Protein Name

 DNA-directed RNA polymerase II subunit RPB11-a 

Protein Synonyms/Alias

 RNA polymerase II subunit B11-a; RPB11a; DNA-directed RNA polymerase II subunit J-1; RNA polymerase II 13.3 kDa subunit 

Gene Name

 POLR2J 

Gene Synonyms/Alias

 POLR2J1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
47	HTLGNIIKSQLLKDP	ubiquitination	[1]
52	IIKSQLLKDPQVLFA	ubiquitination	[1]
62	QVLFAGYKVPHPLEH	ubiquitination	[1]
110	ERFRVAIKDKQEGIE	ubiquitination	[2, 3]
112	FRVAIKDKQEGIE**	ubiquitination	[2, 3]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft (By similarity). 

Sequence Annotation

 MOD_RES 1 1 N-acetylmethionine.  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; DNA-directed RNA polymerase; Nucleus; Reference proteome; Transcription. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 117 AA 

Protein Sequence

Gene Ontology

 GO:0005665; C:DNA-directed RNA polymerase II, core complex; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003899; F:DNA-directed RNA polymerase activity; TAS:ProtInc.
 GO:0006370; P:7-methylguanosine mRNA capping; TAS:Reactome.
 GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome.
 GO:0050434; P:positive regulation of viral transcription; TAS:Reactome.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
 GO:0006283; P:transcription-coupled nucleotide-excision repair; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 

Interpro

 IPR009025; DNA-dir_RNA_pol_RBP11-like.
 IPR008193; RNA_pol_Rpb11_13-16kDa_CS. 

Pfam

  

SMART

  

PROSITE

 PS01154; RNA_POL_L_13KD 

PRINTS