CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031473
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitogen-activated protein kinase 14 
Protein Synonyms/Alias
 cDNA FLJ58214, highly similar to Mitogen-activated protein kinase 14 (EC2.7.11.24) 
Gene Name
 MAPK14 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
44NNIVKCQKLTDDHVQubiquitination[1]
62YQILRGLKYIHSADIubiquitination[1, 2]
75DIIHRDLKPSNLAVNubiquitination[1, 3]
88VNEDCELKILDFGLAubiquitination[1, 3, 4, 5]
156TDHIDQLKLILRLVGubiquitination[1]
171TPGAELLKKISSESAubiquitination[1, 2, 3]
172PGAELLKKISSESARubiquitination[1]
218MLVLDSDKRITAAQAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 283 AA 
Protein Sequence
MKHENVIGLL DVFTPARSLE EFNDVYLVTH LMGADLNNIV KCQKLTDDHV QFLIYQILRG 60
LKYIHSADII HRDLKPSNLA VNEDCELKIL DFGLARHTDD EMTGYVATRW YRAPEIMLNW 120
MHYNQTVDIW SVGCIMAELL TGRTLFPGTD HIDQLKLILR LVGTPGAELL KKISSESARN 180
YIQSLTQMPK MNFANVFIGA NPLAVDLLEK MLVLDSDKRI TAAQALAHAY FAQYHDPDDE 240
PVADPYDQSF ESRDLLIDEW KSLTYDEVIS FVPPPLDQEE MES 283 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004707; F:MAP kinase activity; IEA:InterPro.
 GO:0000165; P:MAPK cascade; IEA:GOC. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR008352; MAPK_p38.
 IPR000719; Prot_kinase_cat_dom. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS50011; PROTEIN_KINASE_DOM 
PRINTS
 PR01773; P38MAPKINASE.