| Tag | Content |
|---|
| CPLM ID | CPLM-003873 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Isocitrate dehydrogenase kinase/phosphatase |
Protein Synonyms/Alias | IDH kinase/phosphatase; IDHK/P |
Gene Name | aceK |
Gene Synonyms/Alias | b4016; JW3976 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 141 | TIPRPLAKDFHPDHG | acetylation | [1] | | 339 | DRVFKVIKDRFAPQK | acetylation | [1] | | 461 | FPGDMLFKNFGVTRH | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation. |
Sequence Annotation | NP_BIND 315 321 ATP (By similarity).
ACT_SITE 371 371 Probable.
BINDING 336 336 ATP (By similarity). |
Keyword | ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glyoxylate bypass; Hydrolase; Kinase; Nucleotide-binding; Protein phosphatase; Reference proteome; Serine/threonine-protein kinase; Transferase; Tricarboxylic acid cycle. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 578 AA |
Protein Sequence | MPRGLELLIA QTILQGFDAQ YGRFLEVTSG AQQRFEQADW HAVQQAMKNR IHLYDHHVGL 60
VVEQLRCITN GQSTDAAFLL RVKEHYTRLL PDYPRFEIAE SFFNSVYCRL FDHRSLTPER 120
LFIFSSQPER RFRTIPRPLA KDFHPDHGWE SLLMRVISDL PLRLRWQNKS RDIHYIIRHL 180
TETLGTDNLA ESHLQVANEL FYRNKAAWLV GKLITPSGTL PFLLPIHQTD DGELFIDTCL 240
TTTAEASIVF GFARSYFMVY APLPAALVEW LREILPGKTT AELYMAIGCQ KHAKTESYRE 300
YLVYLQGCNE QFIEAPGIRG MVMLVFTLPG FDRVFKVIKD RFAPQKEMSA AHVRACYQLV 360
KEHDRVGRMA DTQEFENFVL EKRHISPALM ELLLQEAAEK ITDLGEQIVI RHLYIERRMV 420
PLNIWLEQVE GQQLRDAIEE YGNAIRQLAA ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC 480
YMTEVNFRDI PPPRYPEDEL ASEPWYSVSP GDVFPEEFRH WLCADPRIGP LFEEMHADLF 540
RADYWRALQN RIREGHVEDV YAYRRRQRFS VRYGEMLF 578 |
Gene Ontology | GO:0005737; C:cytoplasm; IC:EcoliWiki. GO:0008772; F:[isocitrate dehydrogenase (NADP+)] kinase activity; IDA:EcoCyc. GO:0016208; F:AMP binding; IDA:EcoCyc. GO:0005524; F:ATP binding; IDA:EcoCyc. GO:0004674; F:protein serine/threonine kinase activity; IDA:EcoCyc. GO:0004722; F:protein serine/threonine phosphatase activity; IDA:EcoCyc. GO:0006006; P:glucose metabolic process; IEA:InterPro. GO:0006097; P:glyoxylate cycle; IC:EcoliWiki. GO:0018105; P:peptidyl-serine phosphorylation; IDA:EcoCyc. GO:0050790; P:regulation of catalytic activity; IDA:EcoCyc. GO:0006099; P:tricarboxylic acid cycle; IC:EcoliWiki. |
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