CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000651
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger ZZ-type and EF-hand domain-containing protein 1 
Protein Synonyms/Alias
  
Gene Name
 ZZEF1 
Gene Synonyms/Alias
 KIAA0399 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
220TMRSSVLKESLDQLVubiquitination[1, 2]
245TRSPEMDKLKSVAKCubiquitination[1]
291CSHWIRLKMKPDVVLubiquitination[1]
526NLLLKYGKPLQLTLQubiquitination[1]
1078GPEGGLRKLDVETWQubiquitination[1]
1251VLSSPLWKPVFRHQVubiquitination[1]
1391WMLEMKQKSLMSLGNubiquitination[1]
1765EDPEKQRKMHMFIARubiquitination[1]
1863YGCYAAKKYSYGHLPubiquitination[1]
2087VFAECSQKRILGLLAubiquitination[1]
2291RAVIVDVKTRKRKTVubiquitination[1]
2490AIYELQMKKTDYFFLubiquitination[3]
2491IYELQMKKTDYFFLEubiquitination[1, 2]
2501YFFLEVQKRFDGDELubiquitination[1, 2, 3, 4, 5, 6, 7, 8, 9]
2525AQRWQPSKSLRLEEQubiquitination[1]
2581ELQQSYAKQRRSKSAubiquitination[1]
2667EEKHEWEKILQKVLQacetylation[10]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [9] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [10] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861
Functional Description
  
Sequence Annotation
 DOMAIN 111 146 EF-hand.
 DOMAIN 226 405 DOC.
 ZN_FING 1777 1825 ZZ-type 1.
 ZN_FING 1826 1872 ZZ-type 2.
 MOD_RES 1464 1464 Phosphoserine (By similarity).
 MOD_RES 1475 1475 Phosphoserine.
 MOD_RES 1509 1509 Phosphoserine.
 MOD_RES 1518 1518 Phosphoserine.
 MOD_RES 1540 1540 Phosphoserine (By similarity).
 MOD_RES 2444 2444 Phosphoserine (By similarity).
 MOD_RES 2667 2667 N6-acetyllysine.
 LIPID 2 2 N-myristoyl glycine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Lipoprotein; Metal-binding; Myristate; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2961 AA 
Protein Sequence
MGNAPSHSSE DEAAAAGGEG WGPHQDWAAV SGTTPGPGVA APALPPAAAL LEPARLREAA 60
AALLPTPPCE SLVSRHRGAL FRWLEERLGR GEESVTLEQF RELLEARGAG CSSEQFEEAF 120
AQFDAEGDGT VDAENMLEAL KNSSGANLQG ELSHIIRQLQ ACSLVPGFTD IFSESKEGLD 180
IHSSMILRFL HRNRLSSAVM PYPMLEHCNN MCTMRSSVLK ESLDQLVQKE KESPGDLTRS 240
PEMDKLKSVA KCYAYIETSS NSADIDKMTN GETSSYWQSD GSACSHWIRL KMKPDVVLRH 300
LSIAVAATDQ SYMPQQVTVA VGRNASDLQE VRDVHIPSNV TGYVTLLENA NVSQLYVQIN 360
IKRCLSDGCD TRIHGLRAVG FQRVKKSGVS VSDASAIWYW SLLTSLVTAS METNPAFVQT 420
VLHNTQKALR HMPPLSLSPG STDFSTFLSP NVLEEVDSFL IRITSCCSTP EVELTLLAFA 480
LARGSVAKVM SSLCTITDHL DTQYDASSLI LSMASVRQNL LLKYGKPLQL TLQACDVKGK 540
EDKSGPENLL VEPWTRDGFL TETGKTRAST IFSTGTESAF QVTQIRIMVR RGGIGAQCGL 600
VFAYNSSSDK FCAEEHFKRF EKYDKWKLQE LRQFVKSRIG CSSDDLGEDD PIGWFELEEE 660
WDEADVKLQQ CRVAKYLMVK FLCTRQESAE RLGVQGLTIS GYLRPARAEA EQSVTCAHCR 720
KDTEESVCGA TLLLRTLQFI QQLAHDLVQQ KESGLKYKSF LDFAGLDLQI FWNFYSKLKQ 780
NPREECVSAQ TLLLQLLQSC FSVLQGDVLA ASEEEKAPIQ SPKGVEAAKE LYTHLCDVVD 840
KVDGDSVPME ILKQEVRNTL LNGAAIFFPN RQTRRNHLFT MMNVTEQEHK QSLQLTFRSL 900
CTYFSDKDPG GLLLLPEKND LAKMNISEVL AVMDTLVSVA ARECELLMLS GAPGEVGSVL 960
FSLFWSVQGS LLSWCYLQLK STDSGAKDLA VDLIEKYVGQ FLASMRAILE SLFSQYSGKT 1020
IVERLCNSVF SMAARQLVIF LLDFCTLDIP HCVLLREFSV LTELLKKLCS GPEGGLRKLD 1080
VETWQQEQPV VLHTWTKESA HNYENNCHEV SVFVSPGATY FEVEFDDRCE TEKRYDYLEF 1140
TDARGRKTRY DTKVGTDKWP KKVTFKAGPR LQFLFHSDSS HNEWGYKFTV TACGLPDVAV 1200
SWGLDLQLLV SRLMGRLASQ CMALKSVRQL GSNMVVPQAK MALVLSSPLW KPVFRHQVCP 1260
ELELEASWPT HPHRNSKEVK NIPDDPCRHF LLDFAQSEPA QNFCGPYSEL FKGFIQACRK 1320
QAPKTDIVAG STIDQAVNAT FAALVYRTPD LYEKLQKYVN SGGKIALSEE FAQVYSLADG 1380
IRIWMLEMKQ KSLMSLGNEA EEKHSSEATE VNPESLAKEC IEKSLLLLKF LPTGISSKES 1440
CEKLETADET SHLQPLNKRQ RTSSVVEEHF QASVSPTEAA PPATGDQSPG LGTQPKLPSS 1500
SGLPAADVSP ATAEEPLSPS TPTRRPPFTR GRLRLLSFRS MEEARLVPTV KEKYPVLKDV 1560
MDFIKDQSLS HRSVVKVLSL RKAQAQSILE VLKITQHCAE SLGQPHCFHP PFILFLLELL 1620
TCQKDFTNYF GHLEGCGADL HKEIRDTYYQ LVLFLVKAVK GFSSLNDRSL LPALSCVQTA 1680
LLHLLDMGWE PNDLAFFVDI QLPDLLMKMS QENISVHDSV ISQWSEEDEL ADAKQNSEWM 1740
DECQDGMFEA WYEKIAQEDP EKQRKMHMFI ARYCDLLNVD ISCDGCDEIA PWHRYRCLQC 1800
SDMDLCKTCF LGGVKPEGHG DDHEMVNMEF TCDHCQGLII GRRMNCNVCD DFDLCYGCYA 1860
AKKYSYGHLP THSITAHPMV TIRISDRQRL IQPYIHNYSW LLFAALALYS AHLASAEDVD 1920
GEKLDPQTRS SATTLRSQCM QLVGDCLMKA HQGKGLKALA LLGVLPDGDS SLEDQALPVT 1980
VPTGASEEQL EKKAVQGAEL SEAGNGKRAV HEEIRPVDFK QRNKADKGVS LSKDPSCQTQ 2040
ISDSPADASP PTGLPDAEDS EVSSQKPIEE KAVTPSPEQV FAECSQKRIL GLLAAMLPPL 2100
KSGPTVPLID LEHVLPLMFQ VVISNAGHLN ETYHLTLGLL GQLIIRLLPA EVDAAVIKVL 2160
SAKHNLFAAG DSSIVPDGWK TTHLLFSLGA VCLDSRVGLD WACSMAEILR SLNSAPLWRD 2220
VIATFTDHCI KQLPFQLKHT NIFTLLVLVG FPQVLCVGTR CVYMDNANEP HNVIILKHFT 2280
EKNRAVIVDV KTRKRKTVKD YQLVQKGGGQ ECGDSRAQLS QYSQHFAFIA SHLLQSSMDS 2340
HCPEAVEATW VLSLALKGLY KTLKAHGFEE IRATFLQTDL LKLLVKKCSK GTGFSKTWLL 2400
RDLEILSIML YSSKKEINAL AEHGDLELDE RGDREEEVER PVSSPGDPEQ KKLDPLEGLD 2460
EPTRICFLMA HDALNAPLHI LRAIYELQMK KTDYFFLEVQ KRFDGDELTT DERIRSLAQR 2520
WQPSKSLRLE EQSAKAVDTD MIILPCLSRP ARCDQATAES NPVTQKLISS TESELQQSYA 2580
KQRRSKSAAL LHKELNCKSK RAVRDYLFRV NEATAVLYAR HVLASLLAEW PSHVPVSEDI 2640
LELSGPAHMT YILDMFMQLE EKHEWEKILQ KVLQGCREDM LGTMALAACQ FMEEPGMEVQ 2700
VRESKHPYNN NTNFEDKVHI PGAIYLSIKF DSQCNTEEGC DELAMSSSSD FQQDRHSFSG 2760
SQQKWKDFEL PGDTLYYRFT SDMSNTEWGY RFTVTAGHLG RFQTGFEILK QMLSEERVVP 2820
HLPLAKIWEW LVGVACRQTG HQRLKAIHLL LRIVRCCGHS DLCDLALLKP LWQLFTHMEY 2880
GLFEDVTQPG ILLPLHRALT ELFFVTENRA QELGVLQDYL LALTTDDHLL RCAAQALQNI 2940
AAISLAINYP NKATRLWNVE C 2961 
Gene Ontology
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR004939; APC_su10/DOC_dom.
 IPR000859; CUB_dom.
 IPR011992; EF-hand-like_dom.
 IPR002048; EF_hand_dom.
 IPR008979; Galactose-bd-like.
 IPR000433; Znf_ZZ. 
Pfam
 PF03256; APC10
 PF00569; ZZ 
SMART
 SM00291; ZnF_ZZ 
PROSITE
 PS51284; DOC
 PS50222; EF_HAND_2
 PS01357; ZF_ZZ_1
 PS50135; ZF_ZZ_2 
PRINTS