CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010617
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA replication licensing factor MCM6 
Protein Synonyms/Alias
 Mis5 homolog 
Gene Name
 Mcm6 
Gene Synonyms/Alias
 Mcmd6; Mis5 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
173KDVEQQFKYTQPNICacetylation[1]
611RDGSGVTKSSWRITVacetylation[2]
643CCDEVQPKHVKEAFRacetylation[2]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. 
Sequence Annotation
 DOMAIN 346 553 MCM.
 NP_BIND 396 403 ATP (Potential).
 MOTIF 528 531 Arginine finger.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 13 13 Phosphoserine (By similarity).
 MOD_RES 271 271 Phosphoserine (By similarity).
 MOD_RES 762 762 Phosphoserine (By similarity).
 MOD_RES 791 791 Phosphothreonine.  
Keyword
 Acetylation; ATP-binding; Cell cycle; Complete proteome; Direct protein sequencing; DNA replication; DNA-binding; Glycoprotein; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 821 AA 
Protein Sequence
MDLAAAAEPG AGSQHPEVRD EVAEKCQKLF LDFLEEFQGS DGEIKYLQFA EELIRPERNT 60
LVVSFADLEQ FNQQLSTTIQ EEFYRVYPYL CRALKTFVKD RKEIPFAKDF YVAFQDLPTR 120
HKIRELTSSR IGLLTRISGQ VVRTHPVHPE LVSGTFLCLD CQTVIKDVEQ QFKYTQPNIC 180
RNPVCANRKR FLLDTNKSRF VDFQKVRIQE TQAELPRGSI PRSLEVILRA EAVESAQAGD 240
RCDFTGALIV VPDVSKLSTP GARAETNSRV SGADGYETEG IRGLRALGVR DLSYRLVFLA 300
CHVAPTNPRF GGKELRDEEQ TAESIKNQMT VKEWEKVFEM SQDKNLYHNL CTSLFPTIHG 360
NDEVKRGVLL MLFGGVPKTT GEGTSLRGDI NVCIVGDPST AKSQFLKHVD EFSPRAVYTS 420
GKASSAAGLT AAVVRDEESH EFVIEAGALM LADNGVCCID EFDKMDMRDQ VAIHEAMEQQ 480
TISITKAGVK ATLNARTSIL AAANPVSGHY DRSKSLKQNI NLSAPIMSRF DLFFILVDEC 540
NEVTDYAIAR RIVDLHSRIE ESIDRVYSLD DIRRYLLFAR QFKPKISKES EDFIVEQYKR 600
LRQRDGSGVT KSSWRITVRQ LESMIRLSES MARMHCCDEV QPKHVKEAFR LLNKSIIRVE 660
TPDVNLDQEE EIQMETDEGQ GGVNGHADSP APVNRFNGSS EDASQETVSK PSLRLGFAEY 720
CRISNLIVLH LRKMEEEEDE SALKRSELVN WYLKEIESEI DSEEELINKK TIIEKVVHRL 780
THYDHVLIEL TQAGLKGSSE GSESYEEDPY LVVNPNYLLE D 821 
Gene Ontology
 GO:0042555; C:MCM complex; ISS:UniProtKB.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004003; F:ATP-dependent DNA helicase activity; IEA:Compara.
 GO:0003697; F:single-stranded DNA binding; IPI:MGI.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0006270; P:DNA replication initiation; IEA:InterPro.
 GO:0006268; P:DNA unwinding involved in replication; IPI:MGI. 
Interpro
 IPR008049; MCM6.
 IPR018525; MCM_CS.
 IPR001208; MCM_DNA-dep_ATPase.
 IPR012340; NA-bd_OB-fold.
 IPR027417; P-loop_NTPase.
 IPR004039; Rubredoxin-type_fold. 
Pfam
 PF00493; MCM 
SMART
 SM00350; MCM 
PROSITE
 PS00847; MCM_1
 PS50051; MCM_2 
PRINTS
 PR01657; MCMFAMILY.
 PR01662; MCMPROTEIN6.