CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015962
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho GTPase-activating protein 30 
Protein Synonyms/Alias
 Rho-type GTPase-activating protein 30 
Gene Name
 ARHGAP30 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
65GVSSNIQKLRQEFESubiquitination[1]
95HCVSSLCKAYFRELPubiquitination[1, 2]
129LEPERLVKILEVLREubiquitination[1]
181APNLLRSKDIEASGFubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 GTPase-activating protein (GAP) for RAC1 and RHOA, but not for CDC42. 
Sequence Annotation
 DOMAIN 20 215 Rho-GAP.
 MOD_RES 996 996 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasmic vesicle; GTPase activation; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1101 AA 
Protein Sequence
MKSRQKGKKK GSAKERVFGC DLQEHLQHSG QEVPQVLKSC AEFVEEYGVV DGIYRLSGVS 60
SNIQKLRQEF ESERKPDLRR DVYLQDIHCV SSLCKAYFRE LPDPLLTYRL YDKFAEAVGV 120
QLEPERLVKI LEVLRELPVP NYRTLEFLMR HLVHMASFSA QTNMHARNLA IVWAPNLLRS 180
KDIEASGFNG TAAFMEVRVQ SIVVEFILTH VDQLFGGAAL SGGEVESGWR SLPGTRASGS 240
PEDLMPRPLP YHLPSILQAG DGPPQMRPYH TIIEIAEHKR KGSLKVRKWR SIFNLGRSGH 300
ETKRKLPRGA EDREDKSNKG TLRPAKSMDS LSAAAGASDE PEGLVGPSSP RPSPLLPESL 360
ENDSIEAAEG EQEPEAEALG GTNSEPGTPR AGRSAIRAGG SSRAERCAGV HISDPYNVNL 420
PLHITSILSV PPNIISNVSL ARLTRGLECP ALQHRPSPAS GPGPGPGLGP GPPDEKLEAS 480
PASSPLADSG PDDLAPALED SLSQEVQDSF SFLEDSSSSE PEWVGAEDGE VAQAEAAGAA 540
FSPGEDDPGM GYLEELLGVG PQVEEFSVEP PLDDLSLDEA QFVLAPSCCS LDSAGPRPEV 600
EEENGEEVFL SAYDDLSPLL GPKPPIWKGS GSLEGEAAGC GRQALGQGGE EQACWEVGED 660
KQAEPGGRLD IREEAEGSPE TKVEAGKASE DRGEAGGSQE TKVRLREGSR EETEAKEEKS 720
KGQKKADSME AKGVEEPGGD EYTDEKEKEI EREEDEQREE AQVEAGRDLE QGAQEDQVAE 780
EKWEVVQKQE AEGVREDEDK GQREKGYHEA RKDQGDGEDS RSPEAATEGG AGEVSKERES 840
GDGEAEGDQR AGGYYLEEDT LSEGSGVASL EVDCAKEGNP HSSEMEEVAP QPPQPEEMEP 900
EGQPSPDGCL CPCSLGLGGV GMRLASTLVQ VQQVRSVPVV PPKPQFAKMP SAMCSKIHVA 960
PANPCPRPGR LDGTPGERAW GSRASRSSWR NGGSLSFDAA VALARDRQRT EAQGVRRTQT 1020
CTEGGDYCLI PRTSPCSMIS AHSPRPLSCL ELPSEGAEGS GSRSRLSLPP REPQVPDPLL 1080
SSQRRSYAFE TQANPGKGEG L 1101 
Gene Ontology
 GO:0016023; C:cytoplasmic membrane-bounded vesicle; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
 GO:0043547; P:positive regulation of GTPase activity; IEA:GOC.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome. 
Interpro
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom. 
Pfam
 PF00620; RhoGAP 
SMART
 SM00324; RhoGAP 
PROSITE
 PS50238; RHOGAP 
PRINTS