CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024629
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitochondrial tRNA-specific 2-thiouridylase 1 
Protein Synonyms/Alias
  
Gene Name
 Trmu 
Gene Synonyms/Alias
 Mtu1 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
106ISCNKHIKFSCFHHYacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2- thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base (By similarity). 
Sequence Annotation
 NP_BIND 10 17 ATP (By similarity).
 REGION 96 98 Interaction with target base in tRNA (By
 REGION 171 173 Interaction with tRNA (By similarity).
 REGION 358 359 Interaction with tRNA (By similarity).
 ACT_SITE 101 101 Nucleophile (By similarity).
 ACT_SITE 222 222 Cysteine persulfide intermediate (By
 BINDING 36 36 ATP; via amide nitrogen and carbonyl
 BINDING 126 126 ATP; via amide nitrogen (By similarity).
 DISULFID 101 222 Alternate (By similarity).  
Keyword
 ATP-binding; Complete proteome; Disulfide bond; Mitochondrion; Nucleotide-binding; Reference proteome; RNA-binding; Transferase; tRNA processing; tRNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 441 AA 
Protein Sequence
MSALRHVVCA LSGGVDSAVA ALLLRRRGYQ VTGVFMKNWD SLDEQGICAA DKDCEDAYKV 60
CQILDIPFHQ VSYVKEYWND VFSDFLNEYE KGRTPNPDIS CNKHIKFSCF HHYAVDNLGA 120
DAVATGHYAR TSLEDEEVFE QKHTKRPDGL FRNRFEVRNP VKLLQAADSF KDQTFFLSQV 180
SQDALRRTIF PLGELTKDFV KKIAAENRLH HVLQKKESMG ICFIGKRNLE HFLLQVSVSD 240
VSGGLLWAGA PVVMKPVFQY LQPRPGKFIS IEDNRVLGTH KGWFLYTLGQ RAKISGLSEP 300
WYVVEKDGTK GDVLVAPRVD HPALYRDLLR TNRVHWIAEE PPAALVRDKM MECHFRFRHQ 360
MALVPCVLTL NQDGTVWVTA VKAVRGLALG QFAVFYKGEE CLGSGKILRL GPSAYTLQKG 420
KNRTRVAPEV SSDSPGLHPT S 441 
Gene Ontology
 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016783; F:sulfurtransferase activity; IEA:InterPro.
 GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
 GO:0008033; P:tRNA processing; IEA:UniProtKB-KW. 
Interpro
 IPR023382; Adenine_a_hdrlase_dom.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR004506; tRNA-specific_2-thiouridylase. 
Pfam
 PF03054; tRNA_Me_trans 
SMART
  
PROSITE
  
PRINTS