UniProt Accession

 UBE2S_RAT; B5DFI8 

Genbank Protein ID

 CH474075; BC169077 

Genbank Nucleotide ID

 EDL75864.1; AAI69077.1 

Protein Name

 Ubiquitin-conjugating enzyme E2 S 

Protein Synonyms/Alias

 Ubiquitin carrier protein S; Ubiquitin-protein ligase S 

Gene Name

 Ube2s 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
206	KHAGERDKKLAAKKK	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination. 

Sequence Annotation

 ACT_SITE 95 95 Glycyl thioester intermediate (By
 MOD_RES 1 1 N-acetylmethionine (By similarity).  

Keyword

 Acetylation; ATP-binding; Cell cycle; Cell division; Complete proteome; Ligase; Nucleotide-binding; Reference proteome; Ubl conjugation; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 223 AA 

Protein Sequence

Gene Ontology

 GO:0005680; C:anaphase-promoting complex; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004842; F:ubiquitin-protein ligase activity; ISS:UniProtKB.
 GO:0051488; P:activation of anaphase-promoting complex activity; ISS:UniProtKB.
 GO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0010458; P:exit from mitosis; ISS:UniProtKB.
 GO:0010994; P:free ubiquitin chain polymerization; ISS:UniProtKB.
 GO:0070979; P:protein K11-linked ubiquitination; ISS:UniProtKB.
 GO:0044314; P:protein K27-linked ubiquitination; ISS:UniProtKB.
 GO:0035519; P:protein K29-linked ubiquitination; ISS:UniProtKB.
 GO:0085020; P:protein K6-linked ubiquitination; ISS:UniProtKB.
 GO:0070534; P:protein K63-linked ubiquitination; ISS:UniProtKB. 

Interpro

 IPR000608; UBQ-conjugat_E2.
 IPR023313; UBQ-conjugating_AS.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF00179; UQ_con 

SMART

  

PROSITE

 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS