CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003471
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ribosomal protein S12 methylthiotransferase RimO 
Protein Synonyms/Alias
 S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO 
Gene Name
 rimO 
Gene Synonyms/Alias
 yliG; b0835; JW0819 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
119HVHHYVPKPKHNPFLacetylation[1]
204SAYGVDVKHRTGFHNacetylation[1]
216FHNGEPVKTSMVSLCacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the methylthiolation of the residue Asp-89 of ribosomal protein S12. 
Sequence Annotation
 DOMAIN 8 118 MTTase N-terminal.
 DOMAIN 376 441 TRAM.
 METAL 17 17 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 53 53 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 82 82 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 150 150 Iron-sulfur (4Fe-4S-S-AdoMet) (By
 METAL 154 154 Iron-sulfur (4Fe-4S-S-AdoMet) (By
 METAL 157 157 Iron-sulfur (4Fe-4S-S-AdoMet) (By  
Keyword
 4Fe-4S; Complete proteome; Cytoplasm; Iron; Iron-sulfur; Metal-binding; Reference proteome; S-adenosyl-L-methionine; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 441 AA 
Protein Sequence
MSKVTPQPKI GFVSLGCPKN LVDSERILTE LRTEGYDVVP SYDDADMVIV NTCGFIDSAV 60
QESLEAIGEA LNENGKVIVT GCLGAKEDQI REVHPKVLEI TGPHSYEQVL EHVHHYVPKP 120
KHNPFLSLVP EQGVKLTPRH YAYLKISEGC NHRCTFCIIP SMRGDLVSRP IGEVLSEAKR 180
LVDAGVKEIL VISQDTSAYG VDVKHRTGFH NGEPVKTSMV SLCEQLSKLG IWTRLHYVYP 240
YPHVDDVIPL MAEGKILPYL DIPLQHASPR ILKLMKRPGS VDRQLARIKQ WREICPELTL 300
RSTFIVGFPG ETEEDFQMLL DFLKEARLDR VGCFKYSPVE GADANALPDQ VPEEVKEERW 360
NRFMQLQQQI SAERLQEKVG REILVIIDEV DEEGAIGRSM ADAPEIDGAV YLNGETNVKP 420
GDILRVKVEH ADEYDLWGSR V 441 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IDA:EcoCyc.
 GO:0035599; F:aspartic acid methylthiotransferase activity; IDA:EcoCyc.
 GO:0005506; F:iron ion binding; IEA:HAMAP.
 GO:0009451; P:RNA modification; IEA:InterPro. 
Interpro
 IPR006638; Elp3/MiaB/NifB.
 IPR023970; MeThioTfrase/rSAM.
 IPR005839; Methylthiotransferase.
 IPR020612; Methylthiotransferase_CS.
 IPR013848; Methylthiotransferase_N.
 IPR012340; NA-bd_OB-fold.
 IPR005840; Ribosomal_S12_MeSTrfase_RimO.
 IPR007197; rSAM.
 IPR023404; rSAM_horseshoe.
 IPR002792; TRAM_dom. 
Pfam
 PF04055; Radical_SAM
 PF00919; UPF0004 
SMART
 SM00729; Elp3 
PROSITE
 PS51449; MTTASE_N
 PS01278; MTTASE_RADICAL
 PS50926; TRAM 
PRINTS