UniProt Accession

 PCNA_RAT; P04961 

Genbank Protein ID

 Y00047; BC060570 

Genbank Nucleotide ID

 CAA68261.1; AAH60570.1 

Protein Name

 Proliferating cell nuclear antigen 

Protein Synonyms/Alias

 PCNA; Cyclin 

Gene Name

 Pcna 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
13	LIQGSILKKVLEALK	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (By similarity). 

Sequence Annotation

 DNA_BIND 61 80 Potential.
 MOD_RES 14 14 N6-acetyllysine (By similarity).
 MOD_RES 77 77 N6-acetyllysine (By similarity).
 MOD_RES 80 80 N6-acetyllysine (By similarity).
 MOD_RES 211 211 Phosphotyrosine; by EGFR (By similarity).
 MOD_RES 248 248 N6-acetyllysine (By similarity).
 CROSSLNK 164 164 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 261 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0015630; C:microtubule cytoskeleton; IEA:Compara.
 GO:0043596; C:nuclear replication fork; IEA:Compara.
 GO:0043626; C:PCNA complex; IEA:InterPro.
 GO:0070557; C:PCNA-p21 complex; ISS:UniProtKB.
 GO:0032139; F:dinucleotide insertion or deletion binding; IEA:Compara.
 GO:0030337; F:DNA polymerase processivity factor activity; IEA:InterPro.
 GO:0000701; F:purine-specific mismatch base pair DNA N-glycosylase activity; IEA:Compara.
 GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
 GO:0007507; P:heart development; IEP:RGD.
 GO:0006298; P:mismatch repair; IEA:Compara.
 GO:0032077; P:positive regulation of deoxyribonuclease activity; ISS:UniProtKB.
 GO:0006275; P:regulation of DNA replication; IEA:InterPro.
 GO:0046686; P:response to cadmium ion; IEP:RGD.
 GO:0033993; P:response to lipid; IDA:RGD.
 GO:0019985; P:translesion synthesis; ISS:UniProtKB. 

Interpro

 IPR000730; Pr_cel_nuc_antig.
 IPR022649; Pr_cel_nuc_antig_C.
 IPR022659; Pr_cel_nuc_antig_CS.
 IPR022648; Pr_cel_nuc_antig_N. 

Pfam

 PF02747; PCNA_C
 PF00705; PCNA_N 

SMART

  

PROSITE

 PS01251; PCNA_1
 PS00293; PCNA_2 

PRINTS

 PR00339; PCNACYCLIN.