CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019628
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Chloride channel CLIC-like protein 1 
Protein Synonyms/Alias
 Mid-1-related chloride channel protein 1 
Gene Name
 CLCC1 
Gene Synonyms/Alias
 KIAA0761; MCLC 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
275FRSSWTYKDDPCQKYubiquitination[1]
313TFVTEPLKHIGKGTGubiquitination[2]
317EPLKHIGKGTGEFIKubiquitination[2]
414TEQGPYAKTYEGRREubiquitination[1, 2, 3, 4, 5, 6, 7, 8, 9]
437LRFQTGNKSPEVLRAubiquitination[1]
495TESSQSAKPVSGQDTubiquitination[2]
514EGSPAAEKAQLKSEAubiquitination[4]
518AAEKAQLKSEAAGSPubiquitination[2, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Seems to act as a chloride ion channel (By similarity). 
Sequence Annotation
 MOD_RES 438 438 Phosphoserine.
 MOD_RES 464 464 Phosphoserine.
 MOD_RES 482 482 Phosphothreonine.
 MOD_RES 509 509 Phosphoserine.
 MOD_RES 524 524 Phosphoserine.
 MOD_RES 532 532 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Endoplasmic reticulum; Golgi apparatus; Membrane; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 551 AA 
Protein Sequence
MLCSLLLCEC LLLVAGYAHD DDWIDPTDML NYDAASGTMR KSQAKYGISG EKDVSPDLSC 60
ADEISECYHK LDSLTYKIDE CEKKKREDYE SQSNPVFRRY LNKILIEAGK LGLPDENKGD 120
MHYDAEIILK RETLLEIQKF LNGEDWKPGA LDDALSDILI NFKFHDFETW KWRFEDSFGV 180
DPYNVLMVLL CLLCIVVLVA TELWTYVRWY TQLRRVLIIS FLFSLGWNWM YLYKLAFAQH 240
QAEVAKMEPL NNVCAKKMDW TGSIWEWFRS SWTYKDDPCQ KYYELLLVNP IWLVPPTKAL 300
AVTFTTFVTE PLKHIGKGTG EFIKALMKEI PALLHLPVLI IMALAILSFC YGAGKSVHVL 360
RHIGGPESEP PQALRPRDRR RQEEIDYRPD GGAGDADFHY RGQMGPTEQG PYAKTYEGRR 420
EILRERDVDL RFQTGNKSPE VLRAFDVPDA EAREHPTVVP SHKSPVLDTK PKETGGILGE 480
GTPKESSTES SQSAKPVSGQ DTSGNTEGSP AAEKAQLKSE AAGSPDQGST YSPARGVAGP 540
RGQDPVSSPC G 551 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IDA:HPA.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. 
Interpro
 IPR009231; Chloride_chnl_CLIC-like. 
Pfam
 PF05934; MCLC 
SMART
  
PROSITE
  
PRINTS