CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007480
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tyrosine-protein kinase CSK 
Protein Synonyms/Alias
 C-Src kinase; Protein-tyrosine kinase MPK-2; p50CSK 
Gene Name
 Csk 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
193SGWALNMKELKLLQTacetylation[1]
196ALNMKELKLLQTIGKacetylation[1]
203KLLQTIGKGEFGDVMacetylation[1]
Reference
 [1] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 
Functional Description
 Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T- cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK (By similarity). 
Sequence Annotation
 DOMAIN 9 70 SH3.
 DOMAIN 82 171 SH2.
 DOMAIN 195 449 Protein kinase.
 NP_BIND 201 209 ATP (By similarity).
 REGION 9 70 Interaction with PTPN8.
 ACT_SITE 314 314 Proton acceptor (By similarity).
 BINDING 222 222 ATP (By similarity).
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 184 184 Phosphotyrosine (By similarity).
 MOD_RES 304 304 Phosphotyrosine (By similarity).
 MOD_RES 364 364 Phosphoserine; by PKA (By similarity).
 MOD_RES 416 416 Phosphotyrosine.  
Keyword
 3D-structure; Acetylation; Adaptive immunity; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Immunity; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; SH2 domain; SH3 domain; Transferase; Tyrosine-protein kinase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 450 AA 
Protein Sequence
MSAIQAAWPS GTECIAKYNF HGTAEQDLPF CKGDVLTIVA VTKDPNWYKA KNKVGREGII 60
PANYVQKREG VKAGTKLSLM PWFHGKITRE QAERLLYPPE TGLFLVREST NYPGDYTLCV 120
SCEGKVEHYR IMYHASKLSI DEEVYFENLM QLVEHYTTDA DGLCTRLIKP KVMEGTVAAQ 180
DEFYRSGWAL NMKELKLLQT IGKGEFGDVM LGDYRGNKVA VKCIKNDATA QAFLAEASVM 240
TQLRHSNLVQ LLGVIVEEKG GLYIVTEYMA KGSLVDYLRS RGRSVLGGDC LLKFSLDVCE 300
AMEYLEGNNF VHRDLAARNV LVSEDNVAKV SDFGLTKEAS STQDTGKLPV KWTAPEALRE 360
KKFSTKSDVW SFGILLWEIY SFGRVPYPRI PLKDVVPRVE KGYKMDAPDG CPPAVYEVMK 420
NCWHLDAATR PTFLQLREQL EHIKTHELHL 450 
Gene Ontology
 GO:0005911; C:cell-cell junction; IDA:MGI.
 GO:0005813; C:centrosome; IEA:Compara.
 GO:0005794; C:Golgi apparatus; IEA:Compara.
 GO:0045121; C:membrane raft; IEA:Compara.
 GO:0005886; C:plasma membrane; TAS:HGNC.
 GO:0005524; F:ATP binding; TAS:HGNC.
 GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; IEA:EC.
 GO:0004713; F:protein tyrosine kinase activity; TAS:HGNC.
 GO:0034332; P:adherens junction organization; IEA:Compara.
 GO:0007420; P:brain development; IEA:Compara.
 GO:0071375; P:cellular response to peptide hormone stimulus; IEA:Compara.
 GO:0035556; P:intracellular signal transduction; TAS:HGNC.
 GO:0045779; P:negative regulation of bone resorption; IEA:Compara.
 GO:0008285; P:negative regulation of cell proliferation; IMP:MGI.
 GO:0070373; P:negative regulation of ERK1 and ERK2 cascade; IEA:Compara.
 GO:0042997; P:negative regulation of Golgi to plasma membrane protein transport; IEA:Compara.
 GO:0032715; P:negative regulation of interleukin-6 production; IEA:Compara.
 GO:0033673; P:negative regulation of kinase activity; IEA:Compara.
 GO:0010989; P:negative regulation of low-density lipoprotein particle clearance; IEA:Compara.
 GO:0050765; P:negative regulation of phagocytosis; IEA:Compara.
 GO:0048709; P:oligodendrocyte differentiation; IEA:Compara.
 GO:0043406; P:positive regulation of MAP kinase activity; IEA:Compara.
 GO:0046777; P:protein autophosphorylation; IEA:Compara.
 GO:0060368; P:regulation of Fc receptor mediated stimulatory signaling pathway; IEA:Compara.
 GO:0050863; P:regulation of T cell activation; TAS:HGNC. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
 IPR000980; SH2.
 IPR001452; SH3_domain.
 IPR008266; Tyr_kinase_AS.
 IPR020635; Tyr_kinase_cat_dom. 
Pfam
 PF07714; Pkinase_Tyr
 PF00017; SH2
 PF00018; SH3_1 
SMART
 SM00252; SH2
 SM00326; SH3
 SM00219; TyrKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00109; PROTEIN_KINASE_TYR
 PS50001; SH2
 PS50002; SH3 
PRINTS
 PR00401; SH2DOMAIN.
 PR00109; TYRKINASE.