UniProt Accession

 NCBP2_DROME; Q9V3L6 

Genbank Protein ID

 AJ238969; AE014297; AY070578 

Genbank Nucleotide ID

 CAB53185.1; AAF55496.1; AAL48049.1 

Protein Name

 Nuclear cap-binding protein subunit 2 

Protein Synonyms/Alias

 20 kDa nuclear cap-binding protein; NCBP 20 kDa subunit; CBP20 

Gene Name

 Cbp20 

Gene Synonyms/Alias

 CG12357 

Created Date

 July 27, 2013 

Organism

 Drosophila melanogaster (Fruit fly) 

NCBI Taxa ID

 7227 

Lysine Modification

Position	Peptide	Type	References
141	AGRGGYGKLLSQKIA	acetylation	[1]

Reference

 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702] 

Functional Description

 Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing and RNA-mediated gene silencing (RNAi). The CBC complex is involved in miRNA-mediated RNA interference via its interaction with Ars2 and is required for primary microRNAs (miRNAs) processing. Also involved in innate immunity via the short interfering RNAs (siRNAs) processing machinery by restricting the viral RNA production. In the CBC complex, Cbp20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires Cbp80 to stabilize the movement of its N- terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. 

Sequence Annotation

 DOMAIN 30 108 RRM.
 REGION 102 106 mRNA cap-binding (By similarity).
 REGION 113 117 mRNA cap-binding (By similarity).
 REGION 123 124 mRNA cap-binding (By similarity).
 BINDING 10 10 mRNA cap (By similarity).
 BINDING 33 33 mRNA cap (By similarity).  

Keyword

 Complete proteome; mRNA capping; mRNA processing; mRNA splicing; Nucleus; Reference proteome; RNA-binding; RNA-mediated gene silencing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 154 AA 

Protein Sequence

Gene Ontology

 GO:0071013; C:catalytic step 2 spliceosome; IDA:FlyBase.
 GO:0005875; C:microtubule associated complex; IDA:FlyBase.
 GO:0005846; C:nuclear cap binding complex; IEA:InterPro.
 GO:0071011; C:precatalytic spliceosome; IDA:FlyBase.
 GO:0003729; F:mRNA binding; ISS:FlyBase.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0000339; F:RNA cap binding; ISS:FlyBase.
 GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-KW.
 GO:0045292; P:mRNA cis splicing, via spliceosome; IEA:InterPro.
 GO:0000398; P:mRNA splicing, via spliceosome; ISS:FlyBase.
 GO:0045071; P:negative regulation of viral genome replication; IMP:FlyBase.
 GO:0031053; P:primary miRNA processing; IMP:UniProtKB.
 GO:0030422; P:production of siRNA involved in RNA interference; IMP:FlyBase. 

Interpro

 IPR027157; NCBP2.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 

Pfam

 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS