CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-042331
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 E3 ubiquitin-protein ligase RFWD2 
Protein Synonyms/Alias
  
Gene Name
 RFWD2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
180SGSSQTKKQPWYNSTubiquitination[1]
233EFQECLSKFTRYNSVubiquitination[1]
464LKLWNVGKPYCLRSFubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Metal-binding; Reference proteome; Repeat; WD repeat; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 566 AA 
Protein Sequence
AYMTKCGHSF CYKCIHQSLE DNNRCPKCNY VVDNIDHLYP NFLVNELILK QKQRFEEKRF 60
KLDHSVSSTN GHRWQIFQDW LGTDQDNLDL ANVNLMLELL VQKKKQLEAE SHAAQLQILM 120
EFLKVARRNK REEMSGLYSP VSEDSTVPQF EAPSPSHSSI IDSTEYSQPP GFSGSSQTKK 180
QPWYNSTLAS RRKRLTAHFE DLEQCYFSTR MSRISDDSRT ASQLDEFQEC LSKFTRYNSV 240
RPLATLSYAS DLYNGSSIVS SIEFDRDCDY FAIAGVTKKI KVYEYDTVIQ DAVDIHYPEN 300
EMTCNSKISC ISWSSYHKNL LASSDYEGTV ILWDGFTGQR SKVYQEHEKR CWSVDFNLMD 360
PKLLASGSDD AKVKLWSTNL DNSVASIEAK ANVCCVKFSP SSRYHLAFGC ADHCVHYYDL 420
RNTKQPIMVF KGHRKAVSYA KFVSGEEIVS ASTDSQLKLW NVGKPYCLRS FKGHINEKNF 480
VGLASNGDYI ACGSENNSLY LYYKGLSKTL LTFKFDTVKS VLDKDRKEDD TNEFVSAVCW 540
RALPDGESNV LIAANSQGTI KVLELV 566 
Gene Ontology
 GO:0005813; C:centrosome; IDA:HPA.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005925; C:focal adhesion; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS. 
Pfam
 PF00400; WD40 
SMART
 SM00184; RING
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS