CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032067
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Next to BRCA1 gene 1 protein 
Protein Synonyms/Alias
 cDNA FLJ55359, highly similar to Next to BRCA1 gene 1 protein 
Gene Name
 NBR1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
87RLAARAGKKPLAHYSubiquitination[1]
88LAARAGKKPLAHYSSubiquitination[1]
151VVNETVEKLEQKLHEubiquitination[1]
253SEPFCHSKYSTPRLPubiquitination[1]
274RLQKQVDKNFLKAEKubiquitination[1]
278QVDKNFLKAEKQRLRubiquitination[1]
307KQLKLHRKIHLWNSIubiquitination[1]
373QPGTKFIKHWRMKNTubiquitination[1]
384MKNTGNVKWSADTKLubiquitination[1]
390VKWSADTKLKFMWGNubiquitination[1]
443SHWRLSHKGQQFGPRubiquitination[1, 2, 3]
478KGMISSSKTDDLTCQubiquitination[1, 3]
494EETFLLAKEERQLGEubiquitination[1, 3, 4]
516TAACIPQKAKNVASEubiquitination[1, 2, 3, 4, 5]
580HDSPLIEKPGLGQIEubiquitination[1]
606PDSMVSVKRKAENIAubiquitination[1]
608SMVSVKRKAENIASVubiquitination[1]
746LERGAEGKPGVEAGQubiquitination[1, 2, 3, 4, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 837 AA 
Protein Sequence
MEPQVTLNVT FKNEIQSFLV SDPENTTWAD IEAMVSINSQ GEYEEALKMA VKQGNQLQMQ 60
VHEGHHVVDE APPPVVGAKR LAARAGKKPL AHYSSLVRVL GSDMKTPEDP AVQSFPLVPC 120
DTDQPQDKPP DWFTSYLETF REQVVNETVE KLEQKLHEKL VLQNPSLGSC PSEVSMPTSE 180
ETLFLPENQF SWHIACNNCQ RRIVGVRYQC SLCPSYNICE DCEAGPYGHD TNHVLLKLRR 240
PVVGSSEPFC HSKYSTPRLP AALEQVRLQK QVDKNFLKAE KQRLRAEKKQ RKAEVKELKK 300
QLKLHRKIHL WNSIHGLQSP KSPLGRPESL LQSNTLMLPL QPCTSVMPML SAAFVDENLP 360
DGTHLQPGTK FIKHWRMKNT GNVKWSADTK LKFMWGNLTL ASTEKKDVLV PCLKAGHVGV 420
VSVEFIAPAL EGTYTSHWRL SHKGQQFGPR VWCSIIVDPF PSEESPDNIE KGMISSSKTD 480
DLTCQQEETF LLAKEERQLG EVTEQTEGTA ACIPQKAKNV ASERELYIPS VDLLTAQDLL 540
SFELLDINIV QELERVPHNT PVDVTPCMSP LPHDSPLIEK PGLGQIEEEN EGAGFKALPD 600
SMVSVKRKAE NIASVEEAEE DLSGTQFVCE TVIRSLTLDA APDHNPPCRQ KSLQMTFALP 660
EGPLGNEKEE IIHIAEEEAV MEEEEDEEDE EEEDELKDEV QSQSSASSED YIIILPECFD 720
TSRPLGDSMY SSALSQPGLE RGAEGKPGVE AGQEPAEAGE RLPGGENQPQ EHSISDILTT 780
SQTLETVPLI PEVVELPPSL PRSSPCVHHH GSPGVDLPVT IPEVSSVPDQ IRGANNF 837 
Gene Ontology
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR000433; Znf_ZZ. 
Pfam
 PF00569; ZZ 
SMART
 SM00291; ZnF_ZZ 
PROSITE
 PS01357; ZF_ZZ_1
 PS50135; ZF_ZZ_2 
PRINTS