CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021215
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Integrin-linked kinase-associated serine/threonine phosphatase 2C 
Protein Synonyms/Alias
 ILKAP 
Gene Name
 ILKAP 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
161HGGIRASKFAAQNLHubiquitination[1, 2]
210LKQASSQKPAWKDGSacetylation[3]
250RYNEESQKHAALSLSubiquitination[4]
258HAALSLSKEHNPTQYubiquitination[4]
373ACNRLANKAVQRGSAubiquitination[5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 
Functional Description
 Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation. 
Sequence Annotation
 DOMAIN 123 390 PP2C-like.
 METAL 152 152 Manganese 1 (By similarity).
 METAL 152 152 Manganese 2 (By similarity).
 METAL 153 153 Manganese 1; via carbonyl oxygen (By
 METAL 326 326 Manganese 2 (By similarity).
 METAL 381 381 Manganese 2 (By similarity).
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 13 13 Phosphoserine.
 MOD_RES 210 210 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Hydrolase; Magnesium; Manganese; Metal-binding; Phosphoprotein; Polymorphism; Protein phosphatase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 392 AA 
Protein Sequence
MDLFGDLPEP ERSPRPAAGK EAQKGPLLFD DLPPASSTDS GSGGPLLFDD LPPASSGDSG 60
SLATSISQMV KTEGKGAKRK TSEEEKNGSE ELVEKKVCKA SSVIFGLKGY VAERKGEREE 120
MQDAHVILND ITEECRPPSS LITRVSYFAV FDGHGGIRAS KFAAQNLHQN LIRKFPKGDV 180
ISVEKTVKRC LLDTFKHTDE EFLKQASSQK PAWKDGSTAT CVLAVDNILY IANLGDSRAI 240
LCRYNEESQK HAALSLSKEH NPTQYEERMR IQKAGGNVRD GRVLGVLEVS RSIGDGQYKR 300
CGVTSVPDIR RCQLTPNDRF ILLACDGLFK VFTPEEAVNF ILSCLEDEKI QTREGKSAAD 360
ARYEAACNRL ANKAVQRGSA DNVTVMVVRI GH 392 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004722; F:protein serine/threonine phosphatase activity; IEA:Compara.
 GO:0045786; P:negative regulation of cell cycle; IEA:Compara.
 GO:0006470; P:protein dephosphorylation; IEA:Compara. 
Interpro
 IPR001932; PP2C-like.
 IPR000222; PP2C_Mn2_Asp60_BS.
 IPR015655; Protein_Pase_2C. 
Pfam
 PF00481; PP2C 
SMART
 SM00331; PP2C_SIG
 SM00332; PP2Cc 
PROSITE
 PS01032; PP2C 
PRINTS