CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031513
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase RIO3 
Protein Synonyms/Alias
 cDNA FLJ54233, highly similar to Serine/threonine-protein kinase RIO3 (EC 2.7.11.1) 
Gene Name
 RIOK3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
86QLRREEKKFNGDSKVubiquitination[1]
92KKFNGDSKVSISFENubiquitination[2, 3, 4, 5]
130DDPYRPAKPVPTPKKubiquitination[6]
142PKKGFIGKGKDITTKubiquitination[4]
144KGFIGKGKDITTKHDubiquitination[4]
149KGKDITTKHDEVVCGubiquitination[3, 4]
191NHVFNALKQHAYSEEubiquitination[2, 3, 4, 5, 7]
274IPTECAIKVFKTTLNubiquitination[2]
277ECAIKVFKTTLNEFKubiquitination[4]
284KTTLNEFKNRDKYIKubiquitination[1, 2, 3, 4, 5, 6, 7]
288NEFKNRDKYIKDDFRubiquitination[4]
291KNRDKYIKDDFRFKDubiquitination[1, 4]
302RFKDRFSKLNPRKIIubiquitination[2, 4, 7]
436NVSQFFQKGGVKEALubiquitination[2, 4, 6]
440FFQKGGVKEALSEREubiquitination[2, 4]
486HVQKNGRKAASFLKDubiquitination[4]
492RKAASFLKDDGDPPLubiquitination[4, 5, 6]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Kinase; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 503 AA 
Protein Sequence
MDLVGVASPE PGTAAAWGPS KCPWAIPQNT ISCSLADVMS EQLAKELQLE EEAAVFPEVA 60
DLMLAQMLQM EYDREYDAQL RREEKKFNGD SKVSISFENY RKVHPYEDSD SSEDEVDWQD 120
TRDDPYRPAK PVPTPKKGFI GKGKDITTKH DEVVCGRKNT ARMENFAPEF QVGDGIGMDL 180
KLSNHVFNAL KQHAYSEERR SARLHEKKEH STAEKAVDPK TRLLMYKMVN SGMLETITGC 240
ISTGKESVVF HAYGGSMEDE KEDSKVIPTE CAIKVFKTTL NEFKNRDKYI KDDFRFKDRF 300
SKLNPRKIIR MWAEKEMHNL ARMQRAGIPC PTVVLLKKHI LVMSFIGHDQ VPAPKLKEVK 360
LNSEEMKEAY YQTLHLMRQL YHECTLVHAD LSEYNMLWHA GKVWLIDVSQ SVEPTHPHGL 420
EFLFRDCRNV SQFFQKGGVK EALSERELFN AVSGLNITAD NEADFLAEIE ALEKMNEDHV 480
QKNGRKAASF LKDDGDPPLL YDE 503 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:InterPro. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR018934; RIO-like_kinase.
 IPR000687; RIO_kinase.
 IPR018935; RIO_kinase_CS.
 IPR017406; Ser/Thr_kinase_Rio3. 
Pfam
 PF01163; RIO1 
SMART
 SM00090; RIO 
PROSITE
 PS01245; RIO1 
PRINTS