CPLM-021716

Genbank Nucleotide ID

Exosome complex component RRP45

Protein Synonyms/Alias

Autoantigen PM/Scl 1; Exosome component 9; P75 polymyositis-scleroderma overlap syndrome-associated autoantigen; Polymyositis/scleroderma autoantigen 1; Polymyositis/scleroderma autoantigen 75 kDa; PM/Scl-75

Mus musculus (Mouse)

Position	Peptide	Type	References
281	RVRKEGGKFGFAESI	acetylation	[1]
425	ANQKAPSKGQGKRKK	acetylation	[2]
429	APSKGQGKRKKKKRT	acetylation	[2, 3]

[1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
[3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]

Functional Description

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC9 binds to ARE-containing RNAs (By similarity).

MOD_RES 297 297 N6-acetyllysine (By similarity).
MOD_RES 306 306 Phosphoserine (By similarity).
MOD_RES 393 393 Phosphoserine (By similarity).
MOD_RES 395 395 Phosphoserine (By similarity).

Acetylation; Complete proteome; Cytoplasm; Exonuclease; Exosome; Hydrolase; Nuclease; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; rRNA processing.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0000178; C:exosome (RNase complex); ISS:UniProtKB.
GO:0005730; C:nucleolus; TAS:MGI.
GO:0017091; F:AU-rich element binding; IEA:Compara.
GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
GO:0043928; P:exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay; IEA:Compara.
GO:0071028; P:nuclear mRNA surveillance; IEA:Compara.
GO:0071035; P:nuclear polyadenylation-dependent rRNA catabolic process; IEA:Compara.
GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC.
GO:0030307; P:positive regulation of cell growth; IEA:Compara.
GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.

IPR001247; ExoRNase_PH_dom1.
IPR015847; ExoRNase_PH_dom2.
IPR020568; Ribosomal_S5_D2-typ_fold.

PF01138; RNase_PH
PF03725; RNase_PH_C