UniProt Accession

 TPA_MOUSE; P11214; Q6P7U0; Q91VP2 

Genbank Protein ID

 J03520; AK135857; AK135965; BC011256; BC057967; BC061508 

Genbank Nucleotide ID

 AAA40470.1; BAE22698.1; BAE22749.1; AAH11256.1; AAH57967.1; AAH61508.1 

Protein Name

 Tissue-type plasminogen activator 

Protein Synonyms/Alias

 t-PA; t-plasminogen activator; tPA; Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B 

Gene Name

 Plat 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
167	ARRPNAIKLGLGNHN	phosphoglycerylation	[1]

Reference

 [1] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237] 

Functional Description

 Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. 

Sequence Annotation

 DOMAIN 36 78 Fibronectin type-I.
 DOMAIN 79 117 EGF-like.
 DOMAIN 124 205 Kringle 1.
 DOMAIN 213 294 Kringle 2.
 DOMAIN 309 558 Peptidase S1.
 REGION 39 49 Important for binding to annexin A2 (By
 ACT_SITE 355 355 Charge relay system.
 ACT_SITE 404 404 Charge relay system.
 ACT_SITE 510 510 Charge relay system.
 CARBOHYD 149 149 N-linked (GlcNAc...) (Potential).
 CARBOHYD 481 481 N-linked (GlcNAc...) (Potential).
 DISULFID 38 68 By similarity.
 DISULFID 66 75 By similarity.
 DISULFID 83 94 By similarity.
 DISULFID 88 105 By similarity.
 DISULFID 107 116 By similarity.
 DISULFID 124 205 By similarity.
 DISULFID 145 187 By similarity.
 DISULFID 176 200 By similarity.
 DISULFID 213 294 By similarity.
 DISULFID 234 276 By similarity.
 DISULFID 265 289 By similarity.
 DISULFID 297 428 Interchain (between A and B chains) (By
 DISULFID 340 356 By similarity.
 DISULFID 348 417 By similarity.
 DISULFID 442 516 By similarity.
 DISULFID 474 490 By similarity.
 DISULFID 506 534 By similarity.  

Keyword

 Cleavage on pair of basic residues; Complete proteome; Direct protein sequencing; Disulfide bond; EGF-like domain; Glycoprotein; Hydrolase; Kringle; Plasminogen activation; Protease; Reference proteome; Repeat; Secreted; Serine protease; Signal; Zymogen. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 559 AA 

Protein Sequence

Gene Ontology

 GO:0045177; C:apical part of cell; IDA:MGI.
 GO:0009986; C:cell surface; IEA:Compara.
 GO:0005615; C:extracellular space; IDA:MGI.
 GO:0030141; C:secretory granule; IDA:MGI.
 GO:0045202; C:synapse; IEA:Compara.
 GO:0004252; F:serine-type endopeptidase activity; IDA:MGI.
 GO:0045861; P:negative regulation of proteolysis; IEA:Compara.
 GO:0031639; P:plasminogen activation; IEA:Compara.
 GO:0048008; P:platelet-derived growth factor receptor signaling pathway; IDA:MGI.
 GO:0060279; P:positive regulation of ovulation; IEA:Compara.
 GO:0006508; P:proteolysis; IDA:MGI.
 GO:0048167; P:regulation of synaptic plasticity; IEA:Compara.
 GO:0051591; P:response to cAMP; IEA:Compara.
 GO:0051384; P:response to glucocorticoid stimulus; IEA:Compara.
 GO:0001666; P:response to hypoxia; IMP:MGI.
 GO:0043434; P:response to peptide hormone stimulus; IEA:Compara.
 GO:0014909; P:smooth muscle cell migration; IMP:MGI.
 GO:0035249; P:synaptic transmission, glutamatergic; IEA:Compara.
 GO:0042060; P:wound healing; IEA:Compara. 

Interpro

 IPR000742; EG-like_dom.
 IPR013032; EGF-like_CS.
 IPR000083; Fibronectin_type1.
 IPR000001; Kringle.
 IPR013806; Kringle-like.
 IPR018056; Kringle_CS.
 IPR001254; Peptidase_S1.
 IPR018114; Peptidase_S1_AS.
 IPR001314; Peptidase_S1A.
 IPR026280; Tissue_plasm_act.
 IPR009003; Trypsin-like_Pept_dom. 

Pfam

 PF00008; EGF
 PF00039; fn1
 PF00051; Kringle
 PF00089; Trypsin 

SMART

 SM00181; EGF
 SM00058; FN1
 SM00130; KR
 SM00020; Tryp_SPc 

PROSITE

 PS00022; EGF_1
 PS01186; EGF_2
 PS50026; EGF_3
 PS01253; FN1_1
 PS51091; FN1_2
 PS00021; KRINGLE_1
 PS50070; KRINGLE_2
 PS50240; TRYPSIN_DOM
 PS00134; TRYPSIN_HIS
 PS00135; TRYPSIN_SER 

PRINTS

 PR00722; CHYMOTRYPSIN.