UniProt Accession

 YIH1_YEAST; P25637; D6VR64 

Genbank Protein ID

 X59720; AY693113; BK006937 

Genbank Nucleotide ID

 CAA42287.1; AAT93132.1; DAA07533.1 

Protein Name

 Protein IMPACT homolog 

Protein Synonyms/Alias

  

Gene Name

 YIH1 

Gene Synonyms/Alias

 YCR059C; YCR59C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
187	VMSAWRIKQDGSAAT	ubiquitination	[1, 2]

Reference

 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Translational regulator that acts under specialized conditions or in a restricted cellular compartment. Acts by interacting with gcn1, thereby preventing activation of gcn2 protein kinase and subsequent down-regulation of protein synthesis. In normal conditions, it resides in a actin complex and has no activity. Under some conditions, it may be displaced from monomeric actin and regulate translation. 

Sequence Annotation

 DOMAIN 10 114 RWD.  

Keyword

 Actin-binding; Complete proteome; Cytoplasm; Nucleus; Reference proteome; Translation regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 258 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0003785; F:actin monomer binding; IDA:SGD.
 GO:0004860; F:protein kinase inhibitor activity; IMP:SGD.
 GO:0043022; F:ribosome binding; IDA:SGD.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW. 

Interpro

 IPR023582; Impact.
 IPR001498; Impact_N.
 IPR020568; Ribosomal_S5_D2-typ_fold.
 IPR006575; RWD-domain.
 IPR016135; UBQ-conjugating_enzyme/RWD.
 IPR020569; UPF0029_Impact_CS. 

Pfam

 PF05773; RWD
 PF01205; UPF0029 

SMART

 SM00591; RWD 

PROSITE

 PS50908; RWD
 PS00910; UPF0029 

PRINTS