UniProt Accession

 EXO5_HUMAN; Q9H790; D3DPV4; Q5SWM7; Q5SWM8; Q5SWM9; Q5SWN0; Q5SWN1; Q8WTW9 

Genbank Protein ID

 AK024797; AL603839; AL603839; AL603839; AL603839; AL603839; AL603839; CH471059; CH471059; CH471059; CH471059; CH471059; BC021969 

Genbank Nucleotide ID

 BAB15008.1; CAI16298.1; CAI16299.1; CAI16300.1; CAI16301.1; CAI16302.1; CAI16303.1; EAX07210.1; EAX07211.1; EAX07212.1; EAX07213.1; EAX07214.1; AAH21969.1 

Protein Name

 Exonuclease V 

Protein Synonyms/Alias

 Exo V; hExo5; Defects in morphology protein 1 homolog 

Gene Name

 EXO5 

Gene Synonyms/Alias

 C1orf176; DEM1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
110	PGFLAPEKAAVLDTG	ubiquitination	[1]
198	ELELAELKTRRRPML	ubiquitination	[2]
243	ASLIHHTKLCLEKPL	ubiquitination	[1]
248	HTKLCLEKPLGPSVL	ubiquitination	[1]
356	ADICEWRKGSGVLSS	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Single-stranded DNA (ssDNA) bidirectional exonuclease involved in DNA repair. Probably involved in DNA repair following ultraviolet (UV) irradiation and interstrand cross-links (ICLs) damage. Has both 5'-3' and 3'-5' exonuclease activities with a strong preference for 5'-ends. Acts as a sliding exonuclease that loads at ssDNA ends and then slides along the ssDNA prior to cutting; however the sliding and the 3'-5' exonuclease activities are abolished upon binding to the replication protein A (RPA) complex that enforces 5'-directionality activity. 

Sequence Annotation

 METAL 92 92 Iron-sulfur (4Fe-4S).
 METAL 343 343 Iron-sulfur (4Fe-4S).
 METAL 346 346 Iron-sulfur (4Fe-4S).
 METAL 352 352 Iron-sulfur (4Fe-4S).  

Keyword

 4Fe-4S; Complete proteome; Cytoplasm; DNA damage; DNA repair; DNA-binding; Exonuclease; Hydrolase; Iron; Iron-sulfur; Magnesium; Metal-binding; Nuclease; Nucleus; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 373 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0042803; F:protein homodimerization activity; TAS:UniProtKB.
 GO:0008310; F:single-stranded DNA specific 3'-5' exodeoxyribonuclease activity; TAS:UniProtKB.
 GO:0045145; F:single-stranded DNA specific 5'-3' exodeoxyribonuclease activity; IDA:UniProtKB.
 GO:0036297; P:interstrand cross-link repair; IMP:UniProtKB. 

Interpro

 IPR019190; EXOV. 

Pfam

 PF09810; Morph_protein1 

SMART

  

PROSITE

  

PRINTS