CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021034
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine beta-lactamase-like protein LACTB, mitochondrial 
Protein Synonyms/Alias
  
Gene Name
 Lactb 
Gene Synonyms/Alias
 Lact1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
180AKLWEAGKLDLDLPVacetylation[1]
223SGIRHYEKDIKKVKEacetylation[1, 2]
232IKKVKEEKAYKALKMacetylation[1, 2]
251PPPSDQEKELKEKGGacetylation[2]
270KSDAPKAKVEQDSEAacetylation[1, 2]
287RSAKPGKKKNDFEQGacetylation[3]
287RSAKPGKKKNDFEQGsuccinylation[3]
288SAKPGKKKNDFEQGEacetylation[3]
288SAKPGKKKNDFEQGEsuccinylation[3]
288SAKPGKKKNDFEQGEsuccinylation[3]
301GELYLKEKFENSIESacetylation[1, 2, 4]
313IESLRLFKNDPLFFKacetylation[3]
313IESLRLFKNDPLFFKsuccinylation[3]
346VERASGYKYLDYMQKacetylation[1, 2, 4, 5]
383ARFYVYNKKKRLVNTacetylation[1, 2, 4]
383ARFYVYNKKKRLVNTphosphoglycerylation[6]
463MSWDKEGKYAMAWGVacetylation[1, 2, 7]
Reference
 [1] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [5] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [6] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237]
 [7] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647
Functional Description
  
Sequence Annotation
 ACT_SITE 162 162 Acyl-ester intermediate (By similarity).
 MOD_RES 162 162 Phosphoserine.
 MOD_RES 264 264 Phosphoserine.
 MOD_RES 346 346 N6-acetyllysine (By similarity).
 MOD_RES 463 463 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Hydrolase; Mitochondrion; Phosphoprotein; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 551 AA 
Protein Sequence
MYRLLSSVTA RAAATAGPAW DGGRRGAHRR PGLPVLGLGW AGGLGLGLGL ALGAKLVVGL 60
RGAVPIQSPA DPEASGTTEL SHEQALSPGS PHTPAPPAAR GFSRAIESSR DLLHRIKDEV 120
GAPGIVVGVS VDGKEVWSEG LGYADVENRV PCKPETVMRI ASISKSLTMV ALAKLWEAGK 180
LDLDLPVQHY VPEFPEKEYE GEKVSVTTRL LISHLSGIRH YEKDIKKVKE EKAYKALKMV 240
KGTPPPSDQE KELKEKGGKN NEKSDAPKAK VEQDSEARCR SAKPGKKKND FEQGELYLKE 300
KFENSIESLR LFKNDPLFFK PGSQFLYSTF GYTLLAAIVE RASGYKYLDY MQKIFHDLDM 360
LTTVQEENEP VIYNRARFYV YNKKKRLVNT PYVDNSYKWA GGGFLSTVGD LLKFGNAMLY 420
GYQVGQFKNS NENLLPGYLK PETMVMMWTP VPNTEMSWDK EGKYAMAWGV VEKKQTYGSC 480
RKQRHYASHT GGAVGASSVL LVLPEELDSE AVNNKVPPRG IIVSIICNMQ SVGLNSTALK 540
IALEFDKDRA D 551 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW. 
Interpro
 IPR001466; Beta-lactam-related.
 IPR012338; Beta-lactam/transpept-like. 
Pfam
 PF00144; Beta-lactamase 
SMART
  
PROSITE
  
PRINTS