CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000310
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein phosphatase with EF-hands 2 
Protein Synonyms/Alias
 PPEF-2 
Gene Name
 PPEF2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
634SWLKNLAKEQLSRENubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 May play a role in phototransduction. May dephosphorylate photoactivated rhodopsin. May function as a calcium sensing regulator of ionic currents, energy production or synaptic transmission. 
Sequence Annotation
 DOMAIN 21 46 IQ.
 DOMAIN 568 603 EF-hand 1.
 DOMAIN 652 687 EF-hand 2.
 DOMAIN 692 727 EF-hand 3.
 REGION 128 540 Catalytic.
 ACT_SITE 241 241 Proton donor (By similarity).
 METAL 179 179 Iron (By similarity).
 METAL 181 181 Iron (By similarity).
 METAL 208 208 Iron (By similarity).
 METAL 208 208 Manganese (By similarity).
 METAL 240 240 Manganese (By similarity).
 METAL 292 292 Manganese (By similarity).
 METAL 488 488 Manganese (By similarity).  
Keyword
 Alternative splicing; Calcium; Cell projection; Cilium; Complete proteome; Cytoplasm; Hydrolase; Iron; Manganese; Metal-binding; Polymorphism; Protein phosphatase; Reference proteome; Repeat; Sensory transduction; Vision. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 753 AA 
Protein Sequence
MGSGTSTQHH FAFQNAERAF KAAALIQRWY RRYVARLEMR RRCTWSIFQS IEYAGQQDQV 60
KLHDFFSYLM DHFIPSSHND RDFLTRIFTE DRFAQDSEMK KCSDYESIEV PDSYTGPRLS 120
FPLLPDHATA LVEAFRLKQQ LHARYVLNLL YETKKHLVQL PNINRVSTCY SEEITVCGDL 180
HGQLDDLIFI FYKNGLPSPE RSYVFNGDFV DRGKDSVEIL MILFAFMLVY PKEFHLNRGN 240
HEDHMVNLRY GFTKEVMNKY KVHGKEILRT LQDVFCWLPL ATLIDEKVLI LHGGVSDITD 300
LELLDKIERS KIVSTMRCKT RQKSEKQMEE KRRANQKSSA QGPIPWFLPE SRSLPSSPLR 360
LGSYKAQKTS RSSSIPCSGS LDGRELSRQV RSSVELELER CRQQAGLLVT GEKEEPSRSA 420
SEADSEAGEL RKPTQEEWRQ VVDILWSDPM AQEGCKANTI RGGGCYFGPD VTQQLLQKYN 480
MQFLIRSHEC KPEGYEFCHN RKVLTIFSAS NYYEVGSNRG AYVKLGPALT PHIVQYQANK 540
VTHTLTMRQR ISRVEESALR ALREKLFAHS SDLLSEFKKH DADKVGLITL SDWAAAVESV 600
LHLGLPWRML RPQLVNSSAD NMLEYKSWLK NLAKEQLSRE NIQSSLLETL YRNRSNLETI 660
FRIIDSDHSG FISLDEFRQT WKLFSSHMNI DITDDCICDL ARSIDFNKDG HIDINEFLEA 720
FRLVEKSCPE GDASECPQAT NAKDSGCSSP GAH 753 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0001917; C:photoreceptor inner segment; IEA:UniProtKB-SubCell.
 GO:0001750; C:photoreceptor outer segment; IEA:UniProtKB-SubCell.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0030544; F:Hsp70 protein binding; IDA:BHF-UCL.
 GO:0051879; F:Hsp90 protein binding; IDA:BHF-UCL.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0030145; F:manganese ion binding; IEA:InterPro.
 GO:0004722; F:protein serine/threonine phosphatase activity; TAS:ProtInc.
 GO:0050906; P:detection of stimulus involved in sensory perception; IEA:InterPro.
 GO:0043409; P:negative regulation of MAPK cascade; IDA:BHF-UCL.
 GO:0010801; P:negative regulation of peptidyl-threonine phosphorylation; IDA:BHF-UCL.
 GO:0006470; P:protein dephosphorylation; TAS:ProtInc.
 GO:0043506; P:regulation of JUN kinase activity; IC:BHF-UCL.
 GO:0007601; P:visual perception; IEA:UniProtKB-KW. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000048; IQ_motif_EF-hand-BS.
 IPR004843; Metallo_PEstase_dom.
 IPR013235; PPP_dom.
 IPR012008; Ser/Thr-Pase_EF-hand_contain.
 IPR006186; Ser/Thr-sp_prot-phosphatase. 
Pfam
 PF13499; EF_hand_5
 PF00149; Metallophos
 PF08321; PPP5 
SMART
 SM00054; EFh
 SM00015; IQ
 SM00156; PP2Ac 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50096; IQ
 PS00125; SER_THR_PHOSPHATASE 
PRINTS
 PR00114; STPHPHTASE.