CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023035
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Calcium-binding mitochondrial carrier protein Aralar2 
Protein Synonyms/Alias
 Citrin; Mitochondrial aspartate glutamate carrier 2; Solute carrier family 25 member 13 
Gene Name
 SLC25A13 
Gene Synonyms/Alias
 ARALAR2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
353VYPIDLVKTRMQNQRubiquitination[1]
408VAPEKAIKLTVNDFVubiquitination[1]
484LGFFGIYKGAKACFLacetylation[2, 3]
484LGFFGIYKGAKACFLubiquitination[1]
487FGIYKGAKACFLRDIubiquitination[1]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle. 
Sequence Annotation
 DOMAIN 51 86 EF-hand 1.
 DOMAIN 87 122 EF-hand 2.
 DOMAIN 125 157 EF-hand 3.
 DOMAIN 158 193 EF-hand 4.
 REPEAT 326 418 Solcar 1.
 REPEAT 426 510 Solcar 2.
 REPEAT 518 606 Solcar 3.
 MOD_RES 484 484 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Calcium; Complete proteome; Disease mutation; Intrahepatic cholestasis; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; Polymorphism; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 675 AA 
Protein Sequence
MAAAKVALTK RADPAELRTI FLKYASIEKN GEFFMSPNDF VTRYLNIFGE SQPNPKTVEL 60
LSGVVDQTKD GLISFQEFVA FESVLCAPDA LFMVAFQLFD KAGKGEVTFE DVKQVFGQTT 120
IHQHIPFNWD SEFVQLHFGK ERKRHLTYAE FTQFLLEIQL EHAKQAFVQR DNARTGRVTA 180
IDFRDIMVTI RPHVLTPFVE ECLVAAAGGT TSHQVSFSYF NGFNSLLNNM ELIRKIYSTL 240
AGTRKDVEVT KEEFVLAAQK FGQVTPMEVD ILFQLADLYE PRGRMTLADI ERIAPLEEGT 300
LPFNLAEAQR QKASGDSARP VLLQVAESAY RFGLGSVAGA VGATAVYPID LVKTRMQNQR 360
STGSFVGELM YKNSFDCFKK VLRYEGFFGL YRGLLPQLLG VAPEKAIKLT VNDFVRDKFM 420
HKDGSVPLAA EILAGGCAGG SQVIFTNPLE IVKIRLQVAG EITTGPRVSA LSVVRDLGFF 480
GIYKGAKACF LRDIPFSAIY FPCYAHVKAS FANEDGQVSP GSLLLAGAIA GMPAASLVTP 540
ADVIKTRLQV AARAGQTTYS GVIDCFRKIL REEGPKALWK GAGARVFRSS PQFGVTLLTY 600
ELLQRWFYID FGGVKPMGSE PVPKSRINLP APNPDHVGGY KLAVATFAGI ENKFGLYLPL 660
FKPSVSTSKA IGGGP 675 
Gene Ontology
 GO:0005887; C:integral to plasma membrane; NAS:UniProtKB.
 GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
 GO:0005509; F:calcium ion binding; IDA:UniProtKB.
 GO:0015183; F:L-aspartate transmembrane transporter activity; IDA:UniProtKB.
 GO:0005313; F:L-glutamate transmembrane transporter activity; IDA:UniProtKB.
 GO:0006754; P:ATP biosynthetic process; IDA:UniProtKB.
 GO:0006094; P:gluconeogenesis; TAS:Reactome.
 GO:0043490; P:malate-aspartate shuttle; IDA:UniProtKB.
 GO:0051592; P:response to calcium ion; IDA:UniProtKB. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR002048; EF_hand_dom.
 IPR002067; Mit_carrier.
 IPR018108; Mitochondrial_sb/sol_carrier.
 IPR023395; Mt_carrier_dom. 
Pfam
 PF13405; EF_hand_4
 PF00153; Mito_carr 
SMART
 SM00054; EFh 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50920; SOLCAR 
PRINTS
 PR00926; MITOCARRIER.