CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016348
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mediator of RNA polymerase II transcription subunit 12-like protein 
Protein Synonyms/Alias
 Mediator complex subunit 12-like protein; Thyroid hormone receptor-associated-like protein; Trinucleotide repeat-containing gene 11 protein-like 
Gene Name
 MED12L 
Gene Synonyms/Alias
 KIAA1635; TNRC11L; TRALP; TRALPUSH; PRO0314 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
82SILAEKLKLNTFQDTubiquitination[1]
432YEVEQQIKQRGRAVEubiquitination[1]
446EVRWSFDKCQESTAGubiquitination[1]
530HRAMAVAKLLEKRQAubiquitination[1]
735ILLYGVGKERDEARHubiquitination[1]
746EARHQLKKITKDILKubiquitination[1]
771GVGDEGQKARKNKQEubiquitination[1]
776GQKARKNKQETFPTLubiquitination[1]
958SSACSKVKQTIYNNVubiquitination[1]
998INYSMLGKILSDNAAubiquitination[1]
1150FLPQATGKPFPGIRSubiquitination[1]
1200NNSVSSLKNDDFTMRubiquitination[1]
1230QNPKSCGKSISIETAubiquitination[1]
1298ICYPHGIKECTEGDNubiquitination[1]
1431SVQGRVLKAAGEELEubiquitination[1]
1457ERDRQKQKSMSLLSQubiquitination[1]
1510ERYQDDIKARQMMHEubiquitination[1]
1650LIDTKGNKIAGFDSIubiquitination[1]
1660GFDSIDKKQGLQVSTubiquitination[1]
1670LQVSTKQKVSPWDLFubiquitination[1]
1851SFVPTNTKQALSNMLubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 May be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). 
Sequence Annotation
 MOD_RES 462 462 Phosphothreonine.
 MOD_RES 1751 1751 Phosphoserine (By similarity).  
Keyword
 Activator; Alternative splicing; Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2145 AA 
Protein Sequence
MAAFGLLSYE QRPLKRPRLG PPDVYPQDPK QKEDELTAVN VKQGFNNQPA FTGDEHGSAR 60
NIVINPSKIG AYFSSILAEK LKLNTFQDTG KKKPQVNAKD NYWLVTARSQ SAIHSWFSDL 120
AGNKPLSILA KKVPILSKKE DVFAYLAKYS VPMVRATWLI KMTCAYYSAI SEAKIKKRQA 180
PDPNLEWTQI STRYLREQLA KISDFYHMAS STGDGPVPVP PEVEQAMKQW EYNEKLAFHM 240
FQEGMLEKHE YLTWILDVLE KIRPMDDDLL KLLLPLMLQY SDEFVQSAYL SRRLAYFCAR 300
RLSLLLSDSP NLLAAHSPHM MIGPNNSSIG APSPGPPGPG MSPVQLAFSD FLSCAQHGPL 360
VYGLSCMLQT VTLCCPSALV WNYSTNENKS ANPGSPLDLL QVAPSSLPMP GGNTAFNQQV 420
RARIYEVEQQ IKQRGRAVEV RWSFDKCQES TAGVTISRVL HTLEVLDRHC FDRTDSSNSM 480
ETLYHKIFWA NQNKDNQEVA PNDEAVVTLL CEWAVSCKRS GKHRAMAVAK LLEKRQAEIE 540
AERCGESEVL DEKESISSSS LAGSSLPVFQ NVLLRFLDTQ APSLSDPNSE CEKVEFVNLV 600
LLFCEFIRHD VFSHDAYMCT LISRGDLSVT ASTRPRSPVG ENADEHYSKD HDVKMEIFSP 660
MPGESCENAN TSLGRRMSVN CEKLVKREKP RELIFPSNYD LLRHLQYATH FPIPLDESSS 720
HECNQRTILL YGVGKERDEA RHQLKKITKD ILKILNKKST TETGVGDEGQ KARKNKQETF 780
PTLETVFTKL QLLSYFDQHQ VTSQISNNVL EQITSFASGT SYHLPLAHHI QLIFDLMEPA 840
LNINGLIDFA IQLLNELSVV EAELLLKSSS LAGSYTTGLC VCIVAVLRRY HSCLILNPDQ 900
TAQVFEGLCG VVKHVVNPSE CSSPERCILA YLYDLYVSCS HLRSKFGDLF SSACSKVKQT 960
IYNNVMPANS NLRWDPDFMM DFIENPSARS INYSMLGKIL SDNAANRYSF VCNTLMNVCM 1020
GHQDAGRIND IANFSSELTA CCTVLSSEWL GVLKALCCSS NHVWGFNDVL CTVDVSDLSF 1080
HDSLATFIAI LIARQCFSLE DVVQHVALPS LLAAACGDAD AEPGARMTCR LLLHLFRAPQ 1140
ACFLPQATGK PFPGIRSSCD RHLLAAAHNS IEVGAVFAVL KAIMMLGDAK IGNNSVSSLK 1200
NDDFTMRGLR CDGNADDIWT ASQNPKSCGK SISIETANLR EYARYVLRTI CQQEWVGEHC 1260
LKEPERLCTD KELILDPVLS NMQAQKLLQL ICYPHGIKEC TEGDNLQRQH IKRILQNLEQ 1320
WTLRQSWLEL QLMIKQCLKD PGSGSVAEMN NLLDNIAKAT IEVFQQSADL NNSSNSGMSL 1380
FNPNSIGSAD TSSTRQNGIK TFLSSSERRG VWLVAPLIAR LPTSVQGRVL KAAGEELEKG 1440
QHLGSSSKKE RDRQKQKSMS LLSQQPFLSL VLTCLKGQDE QREGLLTSLQ NQVNQILSNW 1500
REERYQDDIK ARQMMHEALQ LRLNLVGGMF DTVQRSTQWT TDWALLLLQI ITSGTVDMHT 1560
NNELFTTVLD MLGVLINGTL ASDLSNASPG GSEENKRAYM NLVKKLKKEL GDKRSESIDK 1620
VRQLLPLPKQ TCDVITCEPM GSLIDTKGNK IAGFDSIDKK QGLQVSTKQK VSPWDLFEGQ 1680
KNPAPLSWAW FGTVRVDRRV IKYEEQHHLL LYHTHPMPKP RSYYLQPLPL PPEEEEEEPT 1740
SPVSQEPERK SAELSDQGKT TTDEEKKTKG RKRKTKSSSR VDEYPQSNIY RVPPNYSPIS 1800
SQMMHHPQST LWGYNLVGQP QQPGFFLQNQ SLTPGGSRLD PAGSFVPTNT KQALSNMLQR 1860
RSGAMMQPPS LHAITSQQQL IQMKLLQQQQ QQRLLRQAQT RPFQQGQPGD QAALFAAQAR 1920
PSPQLPQYPG LQQAQTMPQG YTMYGTQMPL QQTSQQQAGS VVLSPSYNSR AYPAAHSNPV 1980
LMERLRQIQQ QPSGYVQQQA SPYLQPLTGS QRLNHQALQQ SPLVGGGIDA VLTSAHPNLP 2040
SVPLPQDPMR PRQPQVRQQQ RLLQMQQPQQ PQPQQPPQPQ QSSQSQSQTL GLQAMQPQQP 2100
LFPRQGLQQT QQQQQTAALV RQLQKQLSSN QPQQGVTPYG HPSHF 2145 
Gene Ontology
 GO:0016592; C:mediator complex; IEA:InterPro.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; IEA:InterPro. 
Interpro
 IPR019035; Mediator_Med12.
 IPR021989; Mediator_Med12_catenin-bd.
 IPR021990; Mediator_Med12_LCEWAV. 
Pfam
 PF09497; Med12
 PF12145; Med12-LCEWAV
 PF12144; Med12-PQL 
SMART
  
PROSITE
  
PRINTS