CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005673
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ETS domain-containing protein Elk-4 
Protein Synonyms/Alias
 Serum response factor accessory protein 1; SAP-1; SRF accessory protein 1 
Gene Name
 ELK4 
Gene Synonyms/Alias
 SAP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
167VKLFKLIKTENPAEKsumoylation[1]
310QDSVLLEKDKVNNSSubiquitination[2]
Reference
 [1] SUMOylation can regulate the activity of ETS-like transcription factor 4.
 Kaikkonen S, Makkonen H, Rytinki M, Palvimo JJ.
 Biochim Biophys Acta. 2010 Aug;1799(8):555-60. [PMID: 20637912]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Involved in both transcriptional activation and repression. Interaction with SIRT7 leads to recruitment and stabilization of SIRT7 at promoters, followed by deacetylation of histone H3 at 'Lys-18' (H3K18Ac) and subsequent transcription repression. Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously. 
Sequence Annotation
 DNA_BIND 5 85 ETS.  
Keyword
 3D-structure; Activator; Alternative splicing; Complete proteome; DNA-binding; Nucleus; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 431 AA 
Protein Sequence
MDSAITLWQF LLQLLQKPQN KHMICWTSND GQFKLLQAEE VARLWGIRKN KPNMNYDKLS 60
RALRYYYVKN IIKKVNGQKF VYKFVSYPEI LNMDPMTVGR IEGDCESLNF SEVSSSSKDV 120
ENGGKDKPPQ PGAKTSSRND YIHSGLYSSF TLNSLNSSNV KLFKLIKTEN PAEKLAEKKS 180
PQEPTPSVIK FVTTPSKKPP VEPVAATISI GPSISPSSEE TIQALETLVS PKLPSLEAPT 240
SASNVMTAFA TTPPISSIPP LQEPPRTPSP PLSSHPDIDT DIDSVASQPM ELPENLSLEP 300
KDQDSVLLEK DKVNNSSRSK KPKGLELAPT LVITSSDPSP LGILSPSLPT ASLTPAFFSQ 360
TPIILTPSPL LSSIHFWSTL SPVAPLSPAR LQGANTLFQF PSVLNSHGPF TLSGLDGPST 420
PGPFSPDLQK T 431 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0003682; F:chromatin binding; IDA:UniProtKB.
 GO:0001047; F:core promoter binding; TAS:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; NAS:ProtInc.
 GO:0003712; F:transcription cofactor activity; TAS:ProtInc.
 GO:0070932; P:histone H3 deacetylation; IDA:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR000418; Ets_dom.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00178; Ets 
SMART
 SM00413; ETS 
PROSITE
 PS00345; ETS_DOMAIN_1
 PS00346; ETS_DOMAIN_2
 PS50061; ETS_DOMAIN_3 
PRINTS
 PR00454; ETSDOMAIN.