UniProt Accession

 TIFA_HUMAN; Q96CG3 

Genbank Protein ID

 AB062110; AB097011; AB097038; AK292900; AC109347; CH471057; BC014259 

Genbank Nucleotide ID

 BAB86902.1; BAC77364.1; BAC77391.1; BAF85589.1; AAY40963.1; EAX06273.1; AAH14259.1 

Protein Name

 TRAF-interacting protein with FHA domain-containing protein A 

Protein Synonyms/Alias

 Putative MAPK-activating protein PM14; Putative NF-kappa-B-activating protein 20; TRAF2-binding protein 

Gene Name

 TIFA 

Gene Synonyms/Alias

 T2BP 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
40	SISFNREKLPSSEVV	ubiquitination	[1]
48	LPSSEVVKFGRNSNI	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
63	CHYTFQDKQVSRVQF	ubiquitination	[1, 3, 4, 7]
77	FSLQLFKKFNSSVLS	ubiquitination	[1]
88	SVLSFEIKNMSKKTN	ubiquitination	[1]
93	EIKNMSKKTNLIVDS	ubiquitination	[1, 7]
108	RELGYLNKMDLPYRC	ubiquitination	[1, 2, 3, 5, 6, 7]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Adapter protein which mediates the IRAK1 and TRAF6 interaction following IL-1 stimulation, resulting in the downstream activation of NF-kappa-B and AP-1 pathways. Induces the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism. 

Sequence Annotation

 DOMAIN 47 103 FHA.  

Keyword

 Complete proteome; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 184 AA 

Protein Sequence

Gene Ontology

 GO:0007249; P:I-kappaB kinase/NF-kappaB cascade; IEA:Compara. 

Interpro

 IPR000253; FHA_dom. 

Pfam

 PF00498; FHA 

SMART

 SM00240; FHA 

PROSITE

 PS50006; FHA_DOMAIN 

PRINTS