CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012148
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NEDD8-activating enzyme E1 regulatory subunit 
Protein Synonyms/Alias
 Amyloid beta precursor protein-binding protein 1, 59 kDa; APP-BP1; Amyloid protein-binding protein 1; Proto-oncogene protein 1 
Gene Name
 NAE1 
Gene Synonyms/Alias
 APPBP1; HPP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*MDAQQTKTNEARLWubiquitination[1]
161GYMRIIIKEHPVIESubiquitination[1]
181LEDLRLDKPFPELREubiquitination[1]
200YDLDHMEKKDHSHTPubiquitination[1]
201DLDHMEKKDHSHTPWubiquitination[1]
229ETNGRIPKTYKEKEDubiquitination[1]
232GRIPKTYKEKEDFRDubiquitination[1]
234IPKTYKEKEDFRDLIubiquitination[1]
247LIRQGILKNENGAPEubiquitination[1]
293DRCINITKQTPSFWIubiquitination[1]
306WILARALKEFVAKEGubiquitination[1]
311ALKEFVAKEGQGNLPubiquitination[1]
332DMIADSGKYIKLQNVubiquitination[1]
335ADSGKYIKLQNVYREubiquitination[1]
357AVGNHVAKLLQSIGQubiquitination[1]
372APESISEKELKLLCSubiquitination[1]
375SISEKELKLLCSNSAubiquitination[1]
450QVEEDIGKLKSCLTGubiquitination[1]
501AAAQEVIKIITKQFVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. 
Sequence Annotation
 REGION 331 344 Interaction with UBA3.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 6 6 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Apoptosis; Cell cycle; Cell membrane; Complete proteome; Membrane; Polymorphism; Reference proteome; Ubl conjugation; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 534 AA 
Protein Sequence
MDAQQTKTNE ARLWGDHGQE ALESAHVCLI NATATGTEIL KNLVLPGIGS FTIIDGNQVS 60
GEDAGNNFFL QRSSIGKNRA EAAMEFLQEL NSDVSGSFVE ESPENLLDND PSFFCRFTVV 120
VATQLPESTS LRLADVLWNS QIPLLICRTY GLVGYMRIII KEHPVIESHP DNALEDLRLD 180
KPFPELREHF QSYDLDHMEK KDHSHTPWIV IIAKYLAQWY SETNGRIPKT YKEKEDFRDL 240
IRQGILKNEN GAPEDEENFE EAIKNVNTAL NTTQIPSSIE DIFNDDRCIN ITKQTPSFWI 300
LARALKEFVA KEGQGNLPVR GTIPDMIADS GKYIKLQNVY REKAKKDAAA VGNHVAKLLQ 360
SIGQAPESIS EKELKLLCSN SAFLRVVRCR SLAEEYGLDT INKDEIISSM DNPDNEIVLY 420
LMLRAVDRFH KQQGRYPGVS NYQVEEDIGK LKSCLTGFLQ EYGLSVMVKD DYVHEFCRYG 480
AAEPHTIAAF LGGAAAQEVI KIITKQFVIF NNTYIYSGMS QTSATFQL 528 
Gene Ontology
 GO:0005737; C:cytoplasm; TAS:ProtInc.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0003824; F:catalytic activity; IEA:InterPro.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0006260; P:DNA replication; IDA:UniProtKB.
 GO:0033314; P:mitotic DNA replication checkpoint; IDA:UniProtKB.
 GO:0045116; P:protein neddylation; IDA:UniProtKB.
 GO:0043523; P:regulation of neuron apoptotic process; IDA:UniProtKB.
 GO:0007165; P:signal transduction; TAS:ProtInc. 
Interpro
 IPR009036; Molybdenum_cofac_synth_MoeB.
 IPR016040; NAD(P)-bd_dom.
 IPR000594; ThiF_NAD_FAD-bd. 
Pfam
 PF00899; ThiF 
SMART
  
PROSITE
  
PRINTS