CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005973
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 cAMP-dependent protein kinase type II-beta regulatory subunit 
Protein Synonyms/Alias
  
Gene Name
 PRKAR2B 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
150NRLQEACKDILLFKNubiquitination[1]
359ELALVTNKPRAASAHubiquitination[1, 2, 3, 4]
372AHAIGTVKCLAMDVQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase. 
Sequence Annotation
 NP_BIND 154 275 cAMP 1.
 NP_BIND 276 418 cAMP 2.
 REGION 2 153 Dimerization and phosphorylation.
 BINDING 223 223 cAMP 1.
 BINDING 232 232 cAMP 1.
 BINDING 352 352 cAMP 2.
 BINDING 361 361 cAMP 2.
 MOD_RES 2 2 N-acetylserine (Probable).
 MOD_RES 83 83 Phosphoserine.
 MOD_RES 85 85 Phosphoserine.
 MOD_RES 114 114 Phosphoserine.  
Keyword
 Acetylation; cAMP; cAMP-binding; Cell membrane; Complete proteome; Cytoplasm; Membrane; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 418 AA 
Protein Sequence
MSIEIPAGLT ELLQGFTVEV LRHQPADLLE FALQHFTRLQ QENERKGTAR FGHEGRTWGD 60
LGAAAGGGTP SKGVNFAEEP MQSDSEDGEE EEAAPADAGA FNAPVINRFT RRASVCAEAY 120
NPDEEEDDAE SRIIHPKTDD QRNRLQEACK DILLFKNLDP EQMSQVLDAM FEKLVKDGEH 180
VIDQGDDGDN FYVIDRGTFD IYVKCDGVGR CVGNYDNRGS FGELALMYNT PRAATITATS 240
PGALWGLDRV TFRRIIVKNN AKKRKMYESF IESLPFLKSL EFSERLKVVD VIGTKVYNDG 300
EQIIAQGDSA DSFFIVESGE VKITMKRKGK SEVEENGAVE IARCSRGQYF GELALVTNKP 360
RAASAHAIGT VKCLAMDVQA FERLLGPCME IMKRNIATYE EQLVALFGTN MDIVEPTA 418 
Gene Ontology
 GO:0005952; C:cAMP-dependent protein kinase complex; IEA:Compara.
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0045121; C:membrane raft; IEA:Compara.
 GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0030552; F:cAMP binding; IEA:UniProtKB-KW.
 GO:0008603; F:cAMP-dependent protein kinase regulator activity; TAS:ProtInc.
 GO:0031625; F:ubiquitin protein ligase binding; IDA:UniProtKB.
 GO:0007202; P:activation of phospholipase C activity; TAS:Reactome.
 GO:0034199; P:activation of protein kinase A activity; TAS:Reactome.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0071377; P:cellular response to glucagon stimulus; TAS:Reactome.
 GO:0006112; P:energy reserve metabolic process; TAS:Reactome.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0006631; P:fatty acid metabolic process; IEA:Compara.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0035556; P:intracellular signal transduction; TAS:ProtInc.
 GO:0007612; P:learning; IEA:Compara.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0050796; P:regulation of insulin secretion; TAS:Reactome.
 GO:0097332; P:response to antipsychotic drug; IEA:Compara.
 GO:0097338; P:response to clozapine; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0055085; P:transmembrane transport; TAS:Reactome.
 GO:0006833; P:water transport; TAS:Reactome. 
Interpro
 IPR002373; cAMP/cGMP_kin.
 IPR012198; cAMP_dep_PK_reg_su.
 IPR003117; cAMP_dep_PK_reg_su_I/II_a/b.
 IPR018490; cNMP-bd-like.
 IPR018488; cNMP-bd_CS.
 IPR000595; cNMP-bd_dom.
 IPR014710; RmlC-like_jellyroll. 
Pfam
 PF00027; cNMP_binding
 PF02197; RIIa 
SMART
 SM00100; cNMP
 SM00394; RIIa 
PROSITE
 PS00888; CNMP_BINDING_1
 PS00889; CNMP_BINDING_2
 PS50042; CNMP_BINDING_3 
PRINTS
 PR00103; CAMPKINASE.