| Tag | Content |
|---|
| CPLM ID | CPLM-007961 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | 6-phosphofructokinase, muscle type |
Protein Synonyms/Alias | Phosphofructo-1-kinase isozyme A; PFK-A; Phosphofructokinase-M; Phosphofructokinase 1; Phosphohexokinase |
Gene Name | Pfkm |
Gene Synonyms/Alias | Pfk-m |
Created Date | July 27, 2013 |
Organism | Rattus norvegicus (Rat) |
NCBI Taxa ID | 10116 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 10 | HEEHHEAKTLGIGKA | acetylation | [1] | | 107 | RAAHNLVKRGITNLC | acetylation | [1] | | 285 | DIKNLVVKRLGYDTR | acetylation | [1] | | 360 | QVTKDVTKAMDEKRF | acetylation | [1] | | 372 | KRFDEAIKLRGRSFM | acetylation | [1] | | 386 | MNNWEVYKLLAHVRP | acetylation | [1] | | 615 | NVEHLVQKMKTTVKR | acetylation | [1] | | 678 | FDRNFATKMGAKATN | acetylation | [1] | | 692 | NWMSGKIKESYRNGR | acetylation | [1] | | 727 | FQPVTELKDQTDFEH | acetylation | [1] | | 738 | DFEHRIPKEQWWLKL | acetylation | [1] | | 744 | PKEQWWLKLRPILKI | acetylation | [1] | | 754 | PILKILAKYEIDLDT | acetylation | [1] |
|
Reference | [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns. Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV. Cell Rep. 2012 Aug 30;2(2):419-31. [ PMID: 22902405] |
Functional Description | Catalyzes the third step of glycolysis, the phosphorylation of fructose-6-phosphate (F6P) by ATP to generate fructose-1,6-bisphosphate (FBP) and ADP (By similarity). |
Sequence Annotation | NP_BIND 35 39 ATP (By similarity).
NP_BIND 193 197 ATP (By similarity).
NP_BIND 210 226 ATP (By similarity).
ACT_SITE 166 166 Proton acceptor (By similarity).
METAL 224 224 Magnesium; via carbonyl oxygen (By
BINDING 201 201 Substrate (By similarity).
BINDING 292 292 Substrate (By similarity).
BINDING 298 298 Substrate (By similarity).
BINDING 301 301 Substrate (By similarity).
MOD_RES 2 2 N-acetylthreonine (By similarity).
MOD_RES 775 775 Phosphoserine (By similarity).
CARBOHYD 530 530 O-linked (GlcNAc...) (By similarity). |
Keyword | Acetylation; Allosteric enzyme; ATP-binding; Complete proteome; Glycolysis; Glycoprotein; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 780 AA |
Protein Sequence | MTHEEHHEAK TLGIGKAIAV LTSGGDAQGM NATVRAVVRV GIFTGLRVFF VHEGYQGLVD 60
GGEHIREATW ESVSMMLQLG GTVIGSARCK DFREREGRLR AAHNLVKRGI TNLCVIGGDG 120
SLTGADTFRS EWSDLLNDLQ KDGKITAEER TKSSYLNIVF LVGSIDNDFC GTDMTIGTDS 180
ALHRIVEIVD AITTTAQSHQ RTFVLEVMGR HCGYLALVTS LSCGADWVFI PECPPDDDWE 240
EHLCRRLSET RTRGSRLNII IVAEGAIDKN GKPITSEDIK NLVVKRLGYD TRVTVLGHVQ 300
RGGTPSAFDR ILGSRMGVEA VMALLEGTPD TPACVVSLSG NTAVRLPLME CVQVTKDVTK 360
AMDEKRFDEA IKLRGRSFMN NWEVYKLLAH VRPPVSKGGL HTVAVMNVGA PAAGMNAAVR 420
STVRIGLIQG NRVLVVHDGF EGLAKGQIEE AGWSYVGGWT GQGGSKLGTK RTLPKKNLEQ 480
ISANITKYNI QGLVIIGGFE AYTGGLELME GRKQFDELCI PFVVIPATVS NNVPGSDFSI 540
GADTALNTIC TTCDRIKQSA AGTKRRVFII ETMGGYCGYL ATMAGLAAGA DAAYIFEEPF 600
TIRDLQVNVE HLVQKMKTTV KRGLVLRNEK CNENYTTDFI FNLYSEEGKG IFDSRKNVLG 660
HMQQGGNPTP FDRNFATKMG AKATNWMSGK IKESYRNGRI FANTPDSGCV LGMRKRALVF 720
QPVTELKDQT DFEHRIPKEQ WWLKLRPILK ILAKYEIDLD TSDHAHLEHI SRKRSGEAAV 780 |
Gene Ontology | GO:0005945; C:6-phosphofructokinase complex; IEA:InterPro. GO:0003872; F:6-phosphofructokinase activity; ISS:UniProtKB. GO:0016208; F:AMP binding; IDA:RGD. GO:0005524; F:ATP binding; IDA:RGD. GO:0030246; F:carbohydrate binding; IDA:RGD. GO:0070095; F:fructose-6-phosphate binding; IDA:RGD. GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. GO:0001678; P:cellular glucose homeostasis; NAS:RGD. GO:0030388; P:fructose 1,6-bisphosphate metabolic process; IDA:RGD. GO:0006002; P:fructose 6-phosphate metabolic process; IDA:RGD. GO:0006096; P:glycolysis; IDA:RGD. GO:0051289; P:protein homotetramerization; IDA:RGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |