UniProt Accession

 SIR4_HUMAN; Q9Y6E7; O43346; Q32M33 

Genbank Protein ID

 AF083109; AC003982; BC109319; BC109320 

Genbank Nucleotide ID

 AAD40852.1; AAB95634.1; AAI09320.1; AAI09321.1 

Protein Name

 NAD-dependent protein deacetylase sirtuin-4 

Protein Synonyms/Alias

 NAD-dependent ADP-ribosyltransferase sirtuin-4; Regulatory protein SIR2 homolog 4; SIR2-like protein 4 

Gene Name

 SIRT4 

Gene Synonyms/Alias

 SIR2L4 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
78	IPDYRSEKVGLYART	ubiquitination	[1]
299	SDDLACLKLNSRCGE	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 NAD-dependent protein ADP-ribosyl transferase. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1. Inhibits GLUD1 enzyme activity. Down- regulates insulin secretion. Has no detectable protein deacetylase activity. 

Sequence Annotation

 DOMAIN 45 314 Deacetylase sirtuin-type.
 NP_BIND 62 82 NAD (By similarity).
 NP_BIND 143 146 NAD (By similarity).
 NP_BIND 260 262 NAD (By similarity).
 NP_BIND 286 288 NAD (By similarity).
 ACT_SITE 161 161 Proton acceptor (By similarity).
 METAL 169 169 Zinc (By similarity).
 METAL 172 172 Zinc (By similarity).
 METAL 220 220 Zinc (By similarity).
 METAL 223 223 Zinc (By similarity).
 BINDING 304 304 NAD; via amide nitrogen (By similarity).  

Keyword

 Complete proteome; Direct protein sequencing; Hydrolase; Metal-binding; Mitochondrion; NAD; Reference proteome; Transferase; Transit peptide; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 314 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; IDA:UniProtKB.
 GO:0016787; F:hydrolase activity; IEA:HAMAP.
 GO:0003950; F:NAD+ ADP-ribosyltransferase activity; IDA:UniProtKB.
 GO:0070403; F:NAD+ binding; IEA:HAMAP.
 GO:0008270; F:zinc ion binding; IEA:HAMAP.
 GO:0006342; P:chromatin silencing; TAS:ProtInc.
 GO:0046676; P:negative regulation of insulin secretion; IMP:UniProtKB.
 GO:0006471; P:protein ADP-ribosylation; TAS:BHF-UCL.
 GO:0006476; P:protein deacetylation; IEA:HAMAP. 

Interpro

 IPR003000; Sirtuin.
 IPR026591; Sirtuin_cat_small_dom.
 IPR026587; Sirtuin_ClassII.
 IPR026590; Ssirtuin_cat_dom. 

Pfam

 PF02146; SIR2 

SMART

  

PROSITE

 PS50305; SIRTUIN 

PRINTS