CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011626
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase HRK1 
Protein Synonyms/Alias
 Hygromycin resistance kinase 1 
Gene Name
 HRK1 
Gene Synonyms/Alias
 YOR267C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
219QKYGKLGKLLGSGAGubiquitination[1]
256RKPNESVKEYAKKCTubiquitination[1]
588ANSDCNDKTDCNANNubiquitination[1, 2]
708RILQVDPKKRATLDDubiquitination[3]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis.
 Ziv I, Matiuhin Y, Kirkpatrick DS, Erpapazoglou Z, Leon S, Pantazopoulou M, Kim W, Gygi SP, Haguenauer-Tsapis R, Reis N, Glickman MH, Kleifeld O.
 Mol Cell Proteomics. 2011 May;10(5):M111.009753. [PMID: 21427232] 
Functional Description
 Involved in regulating the activity of the plasma membrane proton pump PMA1. 
Sequence Annotation
 DOMAIN 215 722 Protein kinase.
 NP_BIND 221 229 ATP (By similarity).
 ACT_SITE 340 340 Proton acceptor (By similarity).
 BINDING 244 244 ATP (By similarity).
 MOD_RES 37 37 Phosphoserine.
 MOD_RES 382 382 Phosphoserine.
 MOD_RES 472 472 Phosphoserine.
 MOD_RES 495 495 Phosphothreonine.
 MOD_RES 498 498 Phosphoserine.  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 759 AA 
Protein Sequence
MPNLLSRNPF HGHHNDHHHD RENSSNNPPQ LIRSSKSFLN FIGRKQSNDS LRSEKSTDSM 60
KSTTTTTNYT TTNLNNNTHS HSNATSISTN NYNNNYETNH HHNISHGLHD YTSPASPKQT 120
HSMAELKRFF RPSVNKKLSM SQLRSKKHST HSPPPSKSTS TVNLNNHYRA QHPHGFTDHY 180
AHTQSAIPPS TDSILSLSNN INIYHDDCIL AQKYGKLGKL LGSGAGGSVK VLVRPTDGAT 240
FAVKEFRPRK PNESVKEYAK KCTAEFCIGS TLHHPNVIET VDVFSDSKQN KYYEVMEYCP 300
IDFFAVVMTG KMSRGEINCC LKQLTEGVKY LHSMGLAHRD LKLDNCVMTS QGILKLIDFG 360
SAVVFRYPFE DGVTMAHGIV GSDPYLAPEV ITSTKSYDPQ CVDIWSIGII YCCMVLKRFP 420
WKAPRDSDDN FRLYCMPDDI EHDYVESARH HEELLKERKE KRQRFLNHSD CSAINQQQPA 480
HESNLKTVQN QVPNTPASIQ GKSDNKPDIV EEETEENKED DSNNDKESTP DNDKESTIDI 540
KISKNENKST VVSANPKKVD ADADADCDAN GDSNGRVDCK ANSDCNDKTD CNANNDCSNE 600
SDCNAKVDTN VNTAANANPD MVPQNNPQQQ QQQQQQQQQQ QQQQQQQHHH HQHQNQDKAH 660
SIASDNKSSQ QHRGPHHKKI IHGPYRLLRL LPHASRPIMS RILQVDPKKR ATLDDIFNDE 720
WFAAIAACTM DSKNKVIRAP GHHHTLVREE NAHLETYKV 759 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; ISS:SGD.
 GO:0006873; P:cellular ion homeostasis; IGI:SGD. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS