CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001256
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Calcium-binding mitochondrial carrier protein Aralar1 
Protein Synonyms/Alias
 Mitochondrial aspartate glutamate carrier 1; Solute carrier family 25 member 12 
Gene Name
 SLC25A12 
Gene Synonyms/Alias
 ARALAR1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
4****MAVKVQTTKRGubiquitination[1, 2]
55NDPNSNPKIVQLLAGubiquitination[3]
250RKDVEVTKEEFAQSAubiquitination[3]
482LGIFGLYKGAKACFLubiquitination[2, 4, 5]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle. 
Sequence Annotation
 DOMAIN 65 76 EF-hand 1.
 DOMAIN 86 121 EF-hand 2.
 DOMAIN 125 155 EF-hand 3.
 DOMAIN 157 192 EF-hand 4.
 REPEAT 324 416 Solcar 1.
 REPEAT 424 508 Solcar 2.
 REPEAT 516 604 Solcar 3.  
Keyword
 Calcium; Complete proteome; Disease mutation; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; Polymorphism; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 678 AA 
Protein Sequence
MAVKVQTTKR GDPHELRNIF LQYASTEVDG ERYMTPEDFV QRYLGLYNDP NSNPKIVQLL 60
AGVADQTKDG LISYQEFLAF ESVLCAPDSM FIVAFQLFDK SGNGEVTFEN VKEIFGQTII 120
HHHIPFNWDC EFIRLHFGHN RKKHLNYTEF TQFLQELQLE HARQAFALKD KSKSGMISGL 180
DFSDIMVTIR SHMLTPFVEE NLVSAAGGSI SHQVSFSYFN AFNSLLNNME LVRKIYSTLA 240
GTRKDVEVTK EEFAQSAIRY GQVTPLEIDI LYQLADLYNA SGRLTLADIE RIAPLAEGAL 300
PYNLAELQRQ QSPGLGRPIW LQIAESAYRF TLGSVAGAVG ATAVYPIDLV KTRMQNQRGS 360
GSVVGELMYK NSFDCFKKVL RYEGFFGLYR GLIPQLIGVA PEKAIKLTVN DFVRDKFTRR 420
DGSVPLPAEV LAGGCAGGSQ VIFTNPLEIV KIRLQVAGEI TTGPRVSALN VLRDLGIFGL 480
YKGAKACFLR DIPFSAIYFP VYAHCKLLLA DENGHVGGLN LLAAGAMAGV PAASLVTPAD 540
VIKTRLQVAA RAGQTTYSGV IDCFRKILRE EGPSAFWKGT AARVFRSSPQ FGVTLVTYEL 600
LQRWFYIDFG GLKPAGSEPT PKSRIADLPP ANPDHIGGYR LATATFAGIE NKFGLYLPKF 660
KSPSVAVVQP KAAVAATQ 678 
Gene Ontology
 GO:0016021; C:integral to membrane; NAS:UniProtKB.
 GO:0005743; C:mitochondrial inner membrane; TAS:Reactome.
 GO:0005509; F:calcium ion binding; IDA:UniProtKB.
 GO:0015183; F:L-aspartate transmembrane transporter activity; IDA:UniProtKB.
 GO:0005313; F:L-glutamate transmembrane transporter activity; IDA:UniProtKB.
 GO:0006094; P:gluconeogenesis; TAS:Reactome.
 GO:0043490; P:malate-aspartate shuttle; IDA:UniProtKB.
 GO:0051592; P:response to calcium ion; IDA:UniProtKB.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR002067; Mit_carrier.
 IPR018108; Mitochondrial_sb/sol_carrier.
 IPR023395; Mt_carrier_dom. 
Pfam
 PF13405; EF_hand_4
 PF00153; Mito_carr 
SMART
 SM00054; EFh 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50920; SOLCAR 
PRINTS
 PR00926; MITOCARRIER.