CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012338
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Inositol 1,4,5-trisphosphate receptor type 2 
Protein Synonyms/Alias
 IP3 receptor isoform 2; IP3R 2; InsP3R2; Type 2 inositol 1,4,5-trisphosphate receptor; Type 2 InsP3 receptor 
Gene Name
 ITPR2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
70MNRYSAQKQYWKAKQubiquitination[1]
91TEAALLKKLQHAAELubiquitination[1]
258LTCDEYEKKQHIFLRubiquitination[1]
450DFANDANKVLATTVKubiquitination[1]
458VLATTVKKLENGTITubiquitination[1]
608NNRKLLEKHITAKEIacetylation[2]
909SYFERLSKFQDGGNNubiquitination[1, 3, 4]
1183SNNYRIVKEILIRLSubiquitination[1]
1589ARSGPRFKEALGGPAubiquitination[1, 5, 6]
2078RELVDVMKNAYNQGLubiquitination[1]
2652EIRSLQEKLESTMSLubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. This release is regulated by cAMP both dependently and independently of PKA (By similarity). 
Sequence Annotation
 DOMAIN 112 166 MIR 1.
 DOMAIN 173 223 MIR 2.
 DOMAIN 231 287 MIR 3.
 DOMAIN 294 372 MIR 4.
 DOMAIN 378 434 MIR 5.
 REGION 265 269 Inositol 1,4,5-trisphosphate binding (By
 REGION 507 510 Inositol 1,4,5-trisphosphate binding (By
 REGION 567 569 Inositol 1,4,5-trisphosphate binding (By
 MOD_RES 937 937 Phosphoserine; by PKA (By similarity).
 MOD_RES 1160 1160 Phosphoserine.
 MOD_RES 1709 1709 Phosphoserine (By similarity).
 MOD_RES 2607 2607 Phosphotyrosine (Potential).  
Keyword
 Alternative splicing; Calcium; Calcium channel; Calcium transport; Complete proteome; Endoplasmic reticulum; Ion channel; Ion transport; Ligand-gated ion channel; Membrane; Phosphoprotein; Polymorphism; Receptor; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2701 AA 
Protein Sequence
MTEKMSSFLY IGDIVSLYAE GSVNGFISTL GLVDDRCVVH PEAGDLANPP KKFRDCLFKV 60
CPMNRYSAQK QYWKAKQAKQ GNHTEAALLK KLQHAAELEQ KQNESENKKL LGEIVKYSNV 120
IQLLHIKSNK YLTVNKRLPA LLEKNAMRVS LDAAGNEGSW FYIHPFWKLR SEGDNIVVGD 180
KVVLMPVNAG QPLHASNIEL LDNPGCKEVN AVNCNTSWKI TLFMKYSSYR EDVLKGGDVV 240
RLFHAEQEKF LTCDEYEKKQ HIFLRTTLRQ SATSATSSKA LWEIEVVHHD PCRGGAGQWN 300
SLFRFKHLAT GNYLAAELNP DYRDAQNEGK NVRDGVPPTS KKKRQAGEKI MYTLVSVPHG 360
NDIASLFELD ATTLQRADCL VPRNSYVRLR HLCTNTWVTS TSIPIDTDEE RPVMLKIGTC 420
QTKEDKEAFA IVSVPLSEVR DLDFANDANK VLATTVKKLE NGTITQNERR FVTKLLEDLI 480
FFVADVPNNG QEVLDVVITK PNRERQKLMR EQNILAQVFG ILKAPFKEKA GEGSMLRLED 540
LGDQRYAPYK YMLRLCYRVL RHSQQDYRKN QEYIAKNFCV MQSQIGYDIL AEDTITALLH 600
NNRKLLEKHI TAKEIETFVS LLRRNREPRF LDYLSDLCVS NTTAIPVTQE LICKFMLSPG 660
NADILIQTKV VSMQADNPME SSILSDDIDD EEVWLYWIDS NKEPHGKAIR HLAQEAKEGT 720
KADLEVLTYY RYQLNLFARM CLDRQYLAIN QISTQLSVDL ILRCVSDESL PFDLRASFCR 780
LMLHMHVDRD PQESVVPVRY ARLWTEIPTK ITIHEYDSIT DSSRNDMKRK FALTMEFVEE 840
YLKEVVNQPF PFGDKEKNKL TFEVVHLARN LIYFGFYSFS ELLRLTRTLL AILDIVQAPM 900
SSYFERLSKF QDGGNNVMRT IHGVGEMMTQ MVLSRGSIFP MSVPDVPPSI HPSKQGSPTE 960
HEDVTVMDTK LKIIEILQFI LSVRLDYRIS YMLSIYKKEF GEDNDNAETS ASGSPDTLLP 1020
SAIVPDIDEI AAQAETMFAG RKEKNPVQLD DEGGRTFLRV LIHLIMHDYP PLLSGALQLL 1080
FKHFSQRAEV LQAFKQVQLL VSNQDVDNYK QIKADLDQLR LTVEKSELWV EKSSNYENGE 1140
IGESQVKGGE EPIEESNILS PVQDGTKKPQ IDSNKSNNYR IVKEILIRLS KLCVQNKKCR 1200
NQHQRLLKNM GAHSVVLDLL QIPYEKNDEK MNEVMNLAHT FLQNFCRGNP QNQVLLHKHL 1260
NLFLTPGLLE AETMRHIFMN NYHLCNEISE RVVQHFVHCI ETHGRHVEYL RFLQTIVKAD 1320
GKYVKKCQDM VMTELINGGE DVLIFYNDRA SFPILLHMMC SERDRGDESG PLAYHITLVE 1380
LLAACTEGKN VYTEIKCNSL LPLDDIVRVV THDDCIPEVK IAYVNFVNHC YVDTEVEMKE 1440
IYTSNHIWKL FENFLVDMAR VCNTTTDRKH ADIFLEKCVT ESIMNIVSGF FNSPFSDNST 1500
SLQTHQPVFI QLLQSAFRIY NCTWPNPAQK ASVESCIRTL AEVAKNRGIA IPVDLDSQVN 1560
TLFMKSHSNM VQRAAMGWRL SARSGPRFKE ALGGPAWDYR NIIEKLQDVV ASLEHQFSPM 1620
MQAEFSVLVD VLYSPELLFP EGSDARIRCG AFMSKLINHT KKLMEKEEKL CIKILQTLRE 1680
MLEKKDSFVE EGNTLRKILL NRYFKGDYSI GVNGHLSGAY SKTAQVGGSF SGQDSDKMGI 1740
SMSDIQCLLD KEGASELVID VIVNTKNDRI FSEGIFLGIA LLEGGNTQTQ YSFYQQLHEQ 1800
KKSEKFFKVL YDRMKAAQKE IRSTVTVNTI DLGNKKRDDD NELMTSGPRM RVRDSTLHLK 1860
EGMKGQLTEA SSATSKAYCV YRREMDPEID IMCTGPEAGN TEEKSAEEVT MSPAIAIMQP 1920
ILRFLQLLCE NHNRELQNFL RNQNNKTNYN LVCETLQFLD CICGSTTGGL GLLGLYINEK 1980
NVALVNQNLE SLTEYCQGPC HENQTCIATH ESNGIDIIIA LILNDINPLG KYRMDLVLQL 2040
KNNASKLLLA IMESRHDSEN AERILFNMRP RELVDVMKNA YNQGLECDHG DDEGGDDGVS 2100
PKDVGHNIYI LAHQLARHNK LLQQMLKPGS DPDEGDEALK YYANHTAQIE IVRHDRTMEQ 2160
IVFPVPNICE YLTRESKCRV FNTTERDEQG SKVNDFFQQT EDLYNEMKWQ KKIRNNPALF 2220
WFSRHISLWG SISFNLAVFI NLAVALFYPF GDDGDEGTLS PLFSVLLWIA VAICTSMLFF 2280
FSKPVGIRPF LVSIMLRSIY TIGLGPTLIL LGAANLCNKI VFLVSFVGNR GTFTRGYRAV 2340
ILDMAFLYHV AYVLVCMLGL FVHEFFYSFL LFDLVYREET LLNVIKSVTR NGRSIILTAV 2400
LALILVYLFS IIGFLFLKDD FTMEVDRLKN RTPVTGSHQV PTMTLTTMME ACAKENCSPT 2460
IPASNTADEE YEDGIERTCD TLLMCIVTVL NQGLRNGGGV GDVLRRPSKD EPLFAARVVY 2520
DLLFYFIVII IVLNLIFGVI IDTFADLRSE KQKKEEILKT TCFICGLERD KFDNKTVSFE 2580
EHIKSEHNMW HYLYFIVLVK VKDPTEYTGP ESYVAQMIVE KNLDWFPRMR AMSLVSNEGD 2640
SEQNEIRSLQ EKLESTMSLV KQLSGQLAEL KEQMTEQRKN KQRLGFLGSN TPHVNHHMPP 2700
H 2701 
Gene Ontology
 GO:0005938; C:cell cortex; IEA:Compara.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; IDA:BHF-UCL.
 GO:0031095; C:platelet dense tubular network membrane; TAS:Reactome.
 GO:0033017; C:sarcoplasmic reticulum membrane; IMP:BHF-UCL.
 GO:0005220; F:inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity; TAS:ProtInc.
 GO:0035091; F:phosphatidylinositol binding; IEA:Compara.
 GO:0007202; P:activation of phospholipase C activity; TAS:Reactome.
 GO:0071320; P:cellular response to cAMP; IEA:Compara.
 GO:0071361; P:cellular response to ethanol; IEA:Compara.
 GO:0006112; P:energy reserve metabolic process; TAS:Reactome.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0038096; P:Fc-gamma receptor signaling pathway involved in phagocytosis; TAS:Reactome.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0050796; P:regulation of insulin secretion; TAS:Reactome.
 GO:0001666; P:response to hypoxia; IDA:BHF-UCL.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR000699; Ca-rel_channel.
 IPR014821; Ins145_P3_rcpt.
 IPR000493; InsP3_rcpt-bd.
 IPR005821; Ion_trans_dom.
 IPR016093; MIR_motif.
 IPR013662; RIH_assoc-dom.
 IPR015925; Ryanodine_recept-rel. 
Pfam
 PF08709; Ins145_P3_rec
 PF00520; Ion_trans
 PF02815; MIR
 PF08454; RIH_assoc
 PF01365; RYDR_ITPR 
SMART
 SM00472; MIR 
PROSITE
 PS50919; MIR 
PRINTS
 PR00779; INSP3RECEPTR.